Role of adenosine in airway inflammation in an allergic mouse model of asthma
Role of adenosine in airway inflammation in an allergic mouse model of asthma
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DOI：
10.1016/j.intimp.2005.07.008
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发表时间：
2006-01-01
影响因子：
5.6
通讯作者：
Mustafa, SJ
中科院分区：
文献类型：
--
作者：
Fan, M;Mustafa, SJ
在本研究中，我们检查了豚草致敏和攻击小鼠在腺苷攻击后支气管肺泡灌洗（BAL）液细胞谱的动态变化。豚草全身致敏和气道攻击的小鼠表现出明显的气道炎症，表现为 BAL 中嗜酸性粒细胞、淋巴细胞、中性粒细胞和活化巨噬细胞增加。腺苷攻击进一步增加了炎症细胞流入 BAL，特别是 1 至 72 小时的中性粒细胞和 1 至 48 小时时间点的嗜酸性粒细胞 (p < 0.05)，在腺苷攻击后的 6 小时时间点急剧上升。腺苷攻击后 1 小时和 72 小时，观察到淋巴细胞更多地浸润到 BAL，巨噬细胞从 6 小时到 72 小时（p < 0.05）。因此，在腺苷攻击后 6 小时时间点分析嗜酸性粒细胞、中性粒细胞和肥大细胞的标记物。腺苷攻击显着增加了 BAL 中嗜酸性粒细胞过氧化物酶、中性粒细胞髓过氧化物酶和己糖胺酶的水平。腺苷激发对肺中肥大细胞脱粒的影响比血液中的肥大细胞脱颗粒更显着。在 BAL 中检测到趋化因子嗜酸细胞趋化因子，腺苷攻击后该趋化因子增加。茶碱是一种非特异性腺苷受体拮抗剂，可防止腺苷增强的炎症细胞及其各自标记物的浸润。我们的研究结果表明，腺苷在过敏性小鼠模型的气道炎症中发挥着重要作用。 (c) 2005 Elsevier B.V. 保留所有权利。
In the present study, we examined dynamic changes in cellular profile of bronchoalveolar lavage (BAL) fluid after adenosine challenge in ragweed sensitized and challenged mice. Mice systemically sensitized and airway challenged with ragweed showed marked airway inflammation manifesting increased eosinophils, lymphocytes, neutrophils and activated macrophages in BAL. Adenosine challenge further enhanced influx of inflammatory cells into BAL, notably neutrophils from 1 to 72 h and eosinophils from 1 to 48 h time-points (p < 0.05), which sharply rose at 6-h time-point following adenosine challenge. Greater infiltration of lymphocytes into BAL was observed at 1 and 72 It and macrophages from 6 to 72 h (p < 0.05) after adenosine challenge. Accordingly, markers of eosinophils, neutrophils and mast cells were analyzed at 6-h time-point after adenosine challenge. Adenosine challenge significantly increased the levels of eosinophil peroxidase, neutrophil myeloperoxidase and hexosaminidase in BAL. There were more significant effects of adenosine challenge on the degranulation of mast cells in the lung than that in blood. The chemoattractant, eotaxin, was detected in BAL, which increased after adenosine challenge. Theophylline, a non-specific adenosine receptor antagonist, prevented adenosine-enhanced infiltration of inflammatory cells and their respective markers. Our findings suggest that adenosine plays an important role in airway inflammation in an allergic mouse model. (c) 2005 Elsevier B.V. All rights reserved.