Antibacterial activity and osseointegration of silver-coated poly(ether ether ketone) prepared using the polydopamine-assisted deposition technique
Antibacterial activity and osseointegration of silver-coated poly(ether ether ketone) prepared using the polydopamine-assisted deposition technique
复制标题
聚多巴胺辅助沉积技术制备的银包聚醚醚酮的抗菌活性和骨整合
DOI：
10.1039/c7tb02436c
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发表时间：
2017-12-21
影响因子：
7
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Gao, Changcheng;Wang, Yong;Li, Bin
聚醚醚酮(PEEK)具有优异的生物相容性和力学性能，是骨科常用的植入材料。然而，种植过程中的细菌感染和无菌性松动会导致许多临床问题，最终可能导致种植失败。因此，赋予种植体抗菌功能，对于促进种植体与骨组织的结合，最终实现种植体的成功植入具有重要作用。本研究旨在利用聚多巴胺(PDA)为基础的表面修饰技术和随后沉积的银纳米颗粒(PEEK-PDA-Ag)来开发一种用于PEEK植入物的生物相容性和抗菌涂层。扫描电子显微镜观察到在PEEK-PDA-Ag表面形成了银纳米颗粒。PEEK-PDA-Ag对MC3T3-E1细胞具有低毒作用。它在体外对金黄色葡萄球菌和大肠杆菌都表现出优异的抗菌性能，在体内也表现出良好的抗菌性能。此外，体内研究表明，PEEK-PDA-Ag植入物具有良好的骨整合，如显微CT评估和推出测试所示。综上所述，本研究结果表明，易于制备的PEEK-PDA-Ag基质具有良好的生物相容性和抗菌性能，有望作为一种有前途的骨科植入材料。
Poly(ether ether ketone) (PEEK) is a popular orthopaedic implant material due to the outstanding biocompatibility and mechanical properties. However, bacterial infections and aseptic loosening during implantation can cause many clinical problems that may eventually lead to implant failure. Therefore, endowing implants with antibacterial functions plays an important role in promoting integration between implants and bone tissue and ultimately, in successful implantation. This study aimed to develop a biocompatible and antibacterial coating for PEEK implants using polydopamine (PDA)-based surface modification technology and subsequent deposition of silver (Ag) nanoparticles (PEEK-PDA-Ag). Formation of Ag nanoparticles was clearly observed on the surface of PEEK-PDA-Ag using scanning electron microscopy. PEEK-PDA-Ag showed low toxicity to MC3T3-E1 cells. It exhibited outstanding antibacterial properties against both S. aureus and E. coli in vitro as well as decent antibacterial performance in vivo. In addition, in vivo studies demonstrated good osseointegration of PEEK-PDA-Ag implants, as shown by micro-CT evaluation and push-out tests. Together, the findings from this study indicate that the facilely prepared PEEK-PDA-Ag substrates possess considerable biocompatibility and antibacterial properties, permitting their potential use as a promising orthopaedic implant material.