Effect of SIVmac infection on plasmacytoid and CD1c+ myeloid dendritic cells in cynomolgus macaques
Effect of SIVmac infection on plasmacytoid and CD1c+ myeloid dendritic cells in cynomolgus macaques
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DOI：
10.1111/j.1365-2567.2007.02758.x
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发表时间：
2008-06-01
期刊：
影响因子：
6.4
通讯作者：
Vaslin, Bruno
中科院分区：
文献类型：
--
作者：
Malleret, Benoit;Karlsson, Ingrid;Vaslin, Bruno
树突状细胞（dc）被认为是诱导和调节免疫反应所必需的。非人类灵长类动物在生物医学研究中至关重要，有助于我们理解dc与人类传染病的关系。然而，目前还没有一种直接的单一平台方法可以对猕猴的DC前体进行定量，从而对血液中这些罕见的细胞群进行精确的绝对血细胞计数。我们采用了一种快速全血测定法，通过四色流式细胞术，使用与人类血液兼容的单平台测定法，对同体猕猴中的dc进行绝对定量。对34只健康猕猴血液中的食蟹猴浆细胞样dc （pDCs）和CD1c（+）髓样dc （CD1c(+) mDCs）进行了定量分析，并与11名健康人的血液样本进行了比较。此外，我们还量化了慢性SIVmac感染食蟹猴体内循环pDCs的绝对数量。感染期间，pDC计数减少，而循环CD1c(+) mDC计数增加。关于非人类灵长类动物中pDC和mDC绝对数量的信息可能会提高我们对这些细胞在SIV/HIV感染和其他传染病中的作用的理解。
Dendritic cells (DCs) are known to be essential for the induction and regulation of immune responses. Non-human primates are essential in biomedical research and contribute to our understanding of the involvement of DCs in human infectious diseases. However, no direct single-platform method for quantifying DC precursors has yet been optimized in macaques to give accurate absolute blood counts of these rare-event cell populations in the blood. We adapted a rapid whole-blood assay for the absolute quantification of DCs in cynomolgous macaques by four-colour flow cytometry, using a single-platform assay compatible with human blood. Cynomolgus macaque plasmacytoid DCs (pDCs) and CD1c(+) myeloid DCs (CD1c(+) mDCs) were quantified in the blood of 34 healthy macaques and the results obtained were compared with those for blood samples from 11 healthy humans. In addition, circulating absolute numbers of pDCs were quantified in cynomolgus macaques chronically infected with SIVmac. During infection, pDC counts decreased whereas circulating CD1c(+) mDC counts increased. Information regarding absolute pDC and mDC counts in non-human primates may improve our understanding of the role of these cells in SIV/HIV infection and in other infectious diseases.