MONOCYTE CHEMOATTRACTANT PROTEIN-1 ACTS AS A T-LYMPHOCYTE CHEMOATTRACTANT
MONOCYTE CHEMOATTRACTANT PROTEIN-1 ACTS AS A T-LYMPHOCYTE CHEMOATTRACTANT
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DOI：
10.1073/pnas.91.9.3652
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发表时间：
1994-04-26
影响因子：
11.1
通讯作者：
SPRINGER, TA
中科院分区：
文献类型：
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作者：
CARR, MW;ROTH, SJ;SPRINGER, TA
我们已经利用了跨内皮淋巴细胞趋化性试验，以确定和纯化有丝分裂原刺激的外周血单核细胞的上清液中的淋巴细胞趋化因子。氨基酸序列分析显示与单核细胞趋化蛋白1（MCP-1），以前被认为是特异性的单核细胞的化学引诱物的身份。重组MCP-1对纯化的T淋巴细胞和外周血淋巴细胞制剂中的CD 3（+）淋巴细胞具有化学吸引力。T细胞对MCP-1的反应是剂量依赖性和趋化性的，而不是趋化动力学的。化学吸引的T淋巴细胞的表型表明它们是激活的记忆亚群。T淋巴细胞对MCP-1的反应可以在缺乏内皮单层的情况下复制，并且有丝分裂原刺激的外周血单核细胞上清液中的大部分T淋巴细胞趋化活性可以被MCP-1抗体中和。因此，MCP-1是由有丝分裂原刺激的外周血单核细胞分泌的主要淋巴细胞化学引诱物，并且能够充当有效的T淋巴细胞以及单核细胞化学引诱物。这可能有助于解释为什么记忆亚群的单核细胞和T淋巴细胞总是一起出现在抗原诱导的炎症部位。
We have utilized a transendothelial lymphocyte chemotaxis assay to identify and purify a lymphocyte chemoattractant in supernatants of mitogen-stimulated peripheral blood mononuclear cells. Amino acid sequence analysis revealed identity with monocyte chemoattractant protein 1 (MCP-1), a chemoattractant previously thought to be specific for monocytes. Recombinant MCP-1 is chemoattractive for purified T lymphocytes and for CD3(+) lymphocytes in peripheral blood lymphocyte preparations. The T-cell response to MCP-1 is dose-dependent and chemotactic, rather than chemokinetic. Phenotyping of chemoattracted T lymphocytes shows they are an activated memory subset. The response to MCP-1 by T lymphocytes can be duplicated in the absence of an endothelial monolayer and the majority of T-lymphocyte chemotactic activity in mitogen-stimulated peripheral blood mononuclear cell supernatants can be neutralized by antibody to MCP-1. Thus, MCP-1 is the major lymphocyte chemoattractant secreted by mitogen-stimulated peripheral blood mononuclear cells and is capable of acting as a potent T-lymphocyte, as well as monocyte, chemoattractant. This may help explain why monocytes and T lymphocytes of the memory subset are always found together at sites of antigen-induced inflammation.