Keratinocyte- and tumor-derived inducers of collagenase.
Keratinocyte- and tumor-derived inducers of collagenase.
复制标题
角质细胞和肿瘤来源的胶原酶诱导剂。
DOI：
10.1111/j.1749-6632.1988.tb18804.x
复制
发表时间：
1988
影响因子：
5.2
通讯作者：
Eisen,AZ
中科院分区：
文献类型：
--
作者：
Bauer,EA;Pentland,AP;Kronberger,A;Wilhelm,SM;Goldberg,GI;Welgus,HG;Eisen,AZ
基底膜区（BMZ）是一个复杂的区域，其中真皮的间质胶原和真皮-表皮界面的蛋白质形成一个复杂的结构大分子网络。BMZ的重塑在形态发生、伤口愈合和肿瘤侵袭中是必需的。由于间质胶原（I型和II型）在结构上的重要性，以及它们在乳头状真皮中与基底膜胶原，特别是含有锚定纤维和锚定斑块的VII型胶原的密切联系，“在该位置间质胶原的裂解可能使整个区域不稳定，如肿瘤浸润”和某些形式的大疱性表皮。上皮细胞对基质成分的调节可能在这一过程中发挥重要作用。对于在形态发生和伤口修复期间发生的事件的一种可能的解释是，上皮释放的因子调节相邻成纤维细胞的胶原酶和/或金属蛋白酶组织抑制剂（TIMP）表达。例如，在兔角膜上皮中发现的细胞因子通过涉及增加的可翻译胶原酶mRNA的存在的机制刺激基质细胞6的胶原酶合成。此外，在人基底细胞癌（BCC）中，胶原酶定位于基质而不是肿瘤岛：这表明肿瘤上皮细胞产生了一种或多种刺激周围皮肤中胶原酶表达的细胞因子。为了探索这种假定的机制，我们采用了从正常人表皮培养的角质形成细胞和提取的基底细胞癌上皮细胞。
The basement membrane zone (BMZ) is a complex region in which the interstitial collagens of the dermis and the proteins of the dermal-epidermal interface form an elaborate network of structural macromolecules. Remodeling of the BMZ is required in morphogenesis, wound healing and tumor invasion. Because of the structural importance of the interstitial collagens (types I and 111) and their close association in the papillary dermis with basement membrane collagens, especially type VII collagencontaining anchoring fibrils and anchoring plaques,'cleavage of the interstitial collagens in that location may destabilize the entire zone, as in tumor invasion" and some forms of epidermolysis bullosa.'Epithelial modulation of stromal elements may play an important role in this process. One possible explanation for the events which occur during morphogenesis and wound repair is that factors released by the epithelium modulate collagenase and/or tissue inhibitor of metalloproteases (TIMP) expression by subadjacent fibroblasts. For example, cytokines found in rabbit corneal epithelium stimulate collagenase synthesis by stromal cells6 through mechanisms involving the presence of increased translatable collagenase mRNA.'Furthermore, in human basal cell carcinomas (BCC), collagenase is localized to the stroma rather than to the tumor islands: suggesting that the tumor epithelium elaborates one or more cytokines which stimulate collagenase expression in surrounding skin To explore this putative mechanism we employed both keratinocytes cultured from normal human epidermis and extracted basal cell carcinoma epithelium.