Structure and expression of Hox-2.2, a murine homeobox-containing gene.
Structure and expression of Hox-2.2, a murine homeobox-containing gene.
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Hox-2.2（一种小鼠同源框基因）的结构和表达。
DOI：
10.1073/pnas.85.15.5582
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发表时间：
1988
影响因子：
11.1
通讯作者：
Ruddle,FH
中科院分区：
文献类型：
--
作者：
Schughart,K;Utset,MF;Awgulewitsch,A;Ruddle,FH
Hox-2.2基因是小鼠11号染色体上一组含有同源框的基因之一。已从成人脊髓文库中分离出含有Hox-2.2同源框的cDNA克隆。Hox-2.2 cDNA和基因组克隆的分析表明，至少有两个oxons和一个内含子。最大的开放阅读框架包括同源框和编码分子量为25，312的224个氨基酸的蛋白质。预测的Hox-2.2蛋白与其他含同源结构域的蛋白质的比较揭示了四个区域的序列相似性：N-末端八肽，同源结构域上游的六肽，同源结构域，和C末端的谷氨酸丰富的区域。这些区域的可能功能进行了讨论。Hox-2.2基因在发育13.5天的胚胎中的后脑和脊髓中表达。比较了Hox-2.2和Hox-2.1在13.5天胚胎中的表达模式。
The Hox-2.2 gene is one of a cluster of homeobox-containing genes on mouse chromosome 11. A cDNA clone containing the Hox-2.2 homeobox has been isolated from an adult spinal cord library. Our analysis of the Hox-2.2 cDNA and genomic clones indicates that there are at least two oxons and one intron. The largest open reading frame includes the homeobox and codes for a 224 amino acid protein of molecular weight 25,312. Comparisons of the predicted Hox-2.2 protein with other homeodomain-containing proteins revealed four regions of sequence similiarity: an N-terminal octapeptide, a hexapeptide upstream of the homeodomain, the homeodomain, and a glutamic acid-rich region at the C terminus. Possible functions of these regions are discussed. The Hox-2.2 gene is expressed in 13.5-day embryos in the developing hindbrain and spinal cord. The expression patterns of Hox-2.2 and Hox-2.1 in 13.5-day embryos are compared.