MOLECULAR ANALYSIS OF THE RECF GENE OF ESCHERICHIA-COLI
MOLECULAR ANALYSIS OF THE RECF GENE OF ESCHERICHIA-COLI
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DOI：
10.1073/pnas.81.15.4622
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
CLARK, AJ
中科院分区：
文献类型：
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作者：
BLANAR, MA;SANDLER, SJ;CLARK, AJ
对野生型E. coli中含有功能性的recF基因和6个转座子Tn3突变，从而分析了recF。分析显示了1071个核苷酸的潜在可翻译的阅读框，其被所有6个插入中断。40.5千道尔顿（kd）的蛋白质将由开放阅读框的翻译产生，并且蛋白质的放射性条带的表观MW为. apprx。通过使用recF+质粒的maxicell方法观察到40 kd。当使用2个recf：：Tn3突变体质粒时，观察到推定的截短肽。注意到来自共同启动子的dnaN和recF的差异表达。recF332：：Tn3转移到染色体上，在半合子条件下，它产生的UV敏感性与2个假定的recF点突变产生的UV敏感性不可区分。
The nucleotide sequence of a 1.325-kilobase reigon of wild-type E. coli containing a functional recF gene and 6 transposon Tn3 mutations that inactivate recF was analyzed. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all 6 insertions. A protein of 40.5 kilodaltons (kd) would result from translation of the open reading frame, and a radioactive band of protein of an apparent MW of .apprx. 40 kd was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when 2 recf::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistiguishable from that produced by 2 presumed recF point mutations.