Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.
Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.
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DOI：
10.1186/s12934-014-0126-z
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发表时间：
2014-09-09
影响因子：
6.4
通讯作者：
Bolivar F
中科院分区：
文献类型：
--
作者：
Rodriguez A;Martínez JA;Flores N;Escalante A;Gosset G;Bolivar F
使用重组微生物发酵工艺生产芳香族氨基酸可以是满足其全球需求的有利方法。此外，大量具有食品和药物应用的化合物可以潜在地从该代谢途径的中间体合成。然而，与其他氨基酸和初级代谢物相反，人工引导构件从中心代谢向芳香族氨基酸途径是复杂的，以有效的方式实现。这一途径的长度和复杂的调节逐渐要求采用更完整的方法，促进互补工具和技术的合并，以克服代谢和调节瓶颈。因此，在过去几年中，已经获得了关于该主题的相关见解，特别是大肠杆菌的转基因菌株。通过将代谢工程策略与合成生物学、系统生物学和生物过程工程的发展相结合，在E.大肠杆菌菌株过量生产芳香族氨基酸，它们的一些前体和相关化合物。在本文中，我们回顾和比较最近成功的报告处理的代谢性状的修改，以达到这些目标。本文的在线版本（doi：10.1186/s12934-014-0126-z）包含补充材料，可供授权用户使用。
The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids and primary metabolites, the artificial channelling of building blocks from central metabolism towards the aromatic amino acid pathway is complicated to achieve in an efficient manner. The length and complex regulation of this pathway have progressively called for the employment of more integral approaches, promoting the merge of complementary tools and techniques in order to surpass metabolic and regulatory bottlenecks. As a result, relevant insights on the subject have been obtained during the last years, especially with genetically modified strains of Escherichia coli. By combining metabolic engineering strategies with developments in synthetic biology, systems biology and bioprocess engineering, notable advances were achieved regarding the generation, characterization and optimization of E. coli strains for the overproduction of aromatic amino acids, some of their precursors and related compounds. In this paper we review and compare recent successful reports dealing with the modification of metabolic traits to attain these objectives. The online version of this article (doi:10.1186/s12934-014-0126-z) contains supplementary material, which is available to authorized users.
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影响因子：
6.4
作者：
Báez-Viveros JL;Flores N;Juárez K;Castillo-España P;Bolivar F;Gosset G
通讯作者：
Gosset G
影响因子：
4.4
作者：
Bartek, Tobias;Blombach, Bastian;Noack, Stephan
通讯作者：
Noack, Stephan
影响因子：
8.4
作者：
Becker, Judith;Zelder, Oskar;Wittmann, Christoph
通讯作者：
Wittmann, Christoph
影响因子：
11.4
作者：
Chen, Kai;Dou, Jie;Zhou, Changlin
通讯作者：
Zhou, Changlin
影响因子：
2.9
作者：
Chandran, SS;Yi, J;Frost, JW
通讯作者：
Frost, JW