Microvasculature of Carotid Atheromatous Plaques: Hemorrhagic Plaques Have Dense Microvessels with Fenestrations to the Arterial Lumen
Microvasculature of Carotid Atheromatous Plaques: Hemorrhagic Plaques Have Dense Microvessels with Fenestrations to the Arterial Lumen
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DOI：
10.1016/j.jstrokecerebrovasdis.2013.12.003
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发表时间：
2014-07-01
影响因子：
2.5
通讯作者：
Masumoto, Junya
中科院分区：
文献类型：
--
作者：
Kurata, Mie;Nose, Masato;Masumoto, Junya
背景资料：众所周知，动脉粥样硬化斑块中的微血管在斑块内出血相关的斑块易损性中发挥作用，但其结构尚不清楚。对人颈动脉粥样硬化斑块（CAPs）的微血管和出血进行形态计量学评价，并对微血管进行三维（3D）重建。方法：对42例患者行颈动脉内膜剥脱术。使用光学显微镜分析标本。斑块出血定义为含有红细胞的区域（>1 mm（2））。为了确定斑块出血的组织病理学特征，将斑块区域分为4个区域：帽、肩、脂质/坏死核心和中膜。然后，定量每个区域中的微血管和巨噬细胞的密度。将两个具有出血性或非出血性斑块的代表性病变切成90个连续切片。切片用抗CD 34和抗α平滑肌肌动蛋白抗体双重染色，使用数字显微镜扫描，并使用TRI-SRF 2软件重建。结果如下：出血性斑块在肩部、冠状和脂质/坏死中心的微血管密度高于非出血性斑块（P分别为0.03、0.009和0.001），其密度与各区域的巨噬细胞呈正相关（P
Background: Microvessels in atheromatous plaques are well known to play a role in plaque vulnerability associated with intraplaque hemorrhage, but their architecture remains unclear. The morphometry of the microvasculature and hemorrhage of human carotid atheromatous plaques (CAPs) were evaluated, and 3-dimensional (3D) reconstruction of the microvessels was performed. Methods: CAPs were obtained by endarterectomy in 42 patients. The specimens were analyzed using light microscopy. Plaque hemorrhage was defined as an area-containing red blood cells (>1 mm(2)). To determine the histopathologic features of plaque hemorrhage, the plaque area was divided into 4 regions: cap, shoulder, lipid/ necrotic core, and media. Then, the density of microvessels and macrophages in each region was quantified. Two representative lesions with either hemorrhagic or nonhemorrhagic plaque were cut into 90 serial sections. The sections were double stained with anti-CD34 and anti-alpha smooth muscle actin antibodies, scanned using a digital microscope, and reconstructed using TRI-SRF2 software. Results: The hemorrhagic plaques showed a higher density of microvessels than nonhemorrhagic plaques in the shoulder, cap, and lipid/ necrotic core (P = .03,.009, and .001, respectively), and there was positive correlations between its density and macrophages in each regions (P