Efficacy of the injectable calcium phosphate ceramics suspensions containing magnesium, zinc and fluoride on the bone mineral deficiency in ovariectornized rats
Efficacy of the injectable calcium phosphate ceramics suspensions containing magnesium, zinc and fluoride on the bone mineral deficiency in ovariectornized rats
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DOI：
10.1002/jps.21131
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发表时间：
2008-01-01
影响因子：
3.8
通讯作者：
Higuchi, William I.
中科院分区：
文献类型：
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作者：
Otsuka, Makoto;Oshinbe, Ayako;Higuchi, William I.
本研究的目的是评估一种新的磷酸钙（CaP）为基础的配方在改善骨质疏松症的卵巢切除（OVX）大鼠的治疗效果。钙磷制剂的离子释放实验对于MZF-CaP（G2：0.46%Mg、5.78%Zn和2.5%F; G3：3.1%Mg、0.03%Zn和3.01%F; G4：1.25%Mg、1.77%Zn、1.35%F）和Zn-TCP（G1：6.17%Zn）粉末，在pH 4.5（37 ° C）的乙酸盐缓冲液中进行MZF-CaP的初始Mg和Zn离子释放速率。Wistar大鼠分为6组，包括正常（非OVX）组（GN）和对照组（GC）。GC组、G1组、G2组、G3组、G4组均行去卵巢。将由CaP制剂（G2、G3、G4）和Zn-TCP粉末（G1）组成的悬浮液注射到除GN和GC之外的所有组中的OVX大鼠的右大腿中，每周一次，持续4周。给GN和GC大鼠注射生理盐水。分析血浆中的锌和碱性磷酸酶水平。采用双能X线骨密度仪（DEXA）测量骨密度（BMD），采用三点弯曲分析法测定骨（股骨）强度。G1和G2组血浆锌水平较高。G1、G2和GN组血浆Zn曲线下面积显著大于G3、G4和GC组（p < 0.05）。第28天，G1、G2、G3和G4组右侧股骨的BMD和骨机械强度显著高于GC组。在G1、G2、G3和G4组中，右侧股骨的BMD和骨机械强度显著高于左侧股骨。然而，G1、G2、G3和G4组之间右侧股骨的BMD无显著差异。结果表明，新的可注射的CaP制剂，是有效的，改善骨质疏松症大鼠的骨性能，并可能是有用的治疗骨质疏松症。(C)2007 Wiley-Liss，Inc.和American Pharmacologist Association J Pharm Sci 97：421-432，2008。
The purpose of this study was to evaluate the therapeutic efficacy of a new calcium phosphate (CaP)-based formulation in improving the bone mineral deficiency in ovariectomized (OVX) rats. The ions release experiments for CaP preparations (G2: 0.46% Mg, 5.78% Zn, and 2.5% F; G3:3.1% Mg, 0.03% Zn, and 3.01% F; G4: 1.25% Mg, 1.77% Zn, 1.35% F) and of a Zn-TCP (G1: 6.17% Zn) powders, the initial Mg and Zn ion release rates of MZF-CaPs were performed in acetate buffer at pH 4.5 (37 degrees C). Wistar rats were divided into six groups including a normal (not OVX) group (GN) and a control, OVX group (GC). Rats in groups GC, G1, G2, G3, G4 were OVX. Suspensions consisting of CaP preparations (G2, G3, G4) and of a Zn-TCP (G1) powders were injected in the right thighs of OVX rats in all groups except for GN and GC, once a week for 4 weeks. GN and GC rats were injected with saline solutions. Plasma was analyzed for Zn land alkaline phosphatase levels. The bone mineral density (BMD) was measured using DEXA and the bone (femur) strength determined using three-point-bending analysis. G1 and G2 groups showed high plasma Zn levels. The area under the curve of plasma Zn was significantly greater in the G1, G2, and GN groups than in the G3, G4, and GC groups (p < 0.05). The BMD and bone mechanical strength of the right femur were significantly higher in the G1, G2, G3, and G4 groups than GC group on day 28. The right femur had significantly greater BMD and bone mechanical strength than the left femur in G1, G2, G3, and G4 groups. However, there was no significant difference in the BMD of the right femur between the G1, G2, G3, and G4 groups. Results indicate that the new injectable CaP formulations, are effective in improving bone properties of OVX rats and may be useful in osteoporosis therapy. (C) 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:421-432, 2008.