Two-dimensional gel analyses of the 24-kDa cap binding protein from poliovirus-infected and uninfected HeLa cells.
Two-dimensional gel analyses of the 24-kDa cap binding protein from poliovirus-infected and uninfected HeLa cells.
复制标题
对来自脊髓灰质炎病毒感染和未感染的 HeLa 细胞的 24 kDa 帽结合蛋白进行二维凝胶分析。
DOI：
10.1016/0042-6822(86)90155-8
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Ehrenfeld,E
中科院分区：
文献类型：
--
作者：
Buckley,B;Ehrenfeld,E
用m7GTP亲和层析从未感染、模拟感染和脊髓灰质炎病毒感染的HeLa细胞提取液中分离得到24 kDa的帽结合蛋白(CBP)，并用等电聚焦和SDS-PAGE进行检测。在所有病例中均发现两个主要种(pI6.7和7.1)和两个次要种(pI16.5和6.S)。从发情后乳房上清液(S200)和0.5MKCl洗涤的核糖体(RSW)上清液中也发现了这四种细菌。我们的结论是，在核糖体相关的24-kDa CBP和可溶性24-kDa CBP之间没有可检测到的差异，并且24-kDa的IMP不会被脊髓灰质炎病毒感染检测到改变。我们还报告了一个先前未被描述的16 kDa多肽(S)的存在，它与来自未感染、模拟感染和脊髓灰质炎病毒感染的HeLa细胞的24 kDa CBP相互作用。
The 24-kDa cap binding protein (CBP) from uninfected, mock-infected and poliovirus-infected HeLa cell extracts was isolated by m7GTP affinity chromatography and examined by isoelectric focusing followed by SDS-PAGE. Two major species (pI6.7 and 7.1) and two minor species pI16.5 and 6.S) were found in all cases. Preparations from postri bosomal supernate (S200) and the supernate from 0.5MKCl washed ribosomes (RSW) also demonstrated these same four species. We conclude that there are no detectable ditferences between ribosome-associated and soluble 24-kDa CBP and that the 24-kDa IMP is not detectably altered by poliovirus infection. We also report the presence of a previously undescribed 16-kDa polypeptide(s) doublet that copurifies with the 24-kDa CBP from uninfected, mock-infected and poliovirus-infected HeLa cells.