Drechslera teres–infected barley (Hordeum vulgare L.) leaves accumulate eight isoforms of thaumatin-like proteins
Drechslera teres–infected barley (Hordeum vulgare L.) leaves accumulate eight isoforms of thaumatin-like proteins
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DOI：
10.1006/pmpp.2001.0325
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发表时间：
2001-04
影响因子：
2.7
通讯作者：
E. Reiss;C. Horstmann
中科院分区：
文献类型：
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作者：
E. Reiss;C. Horstmann
摘要大麦（Hordeum vulgare L.）通过双向电泳和N-末端微测序，在Drechslera teres感染的叶片中检测到4种蛋白质（TLP 1-TLP 8），其中4种为酸性蛋白，4种为碱性蛋白。部分氨基酸序列数据被用来产生聚合酶链反应（PCR）克隆用于分离的四个新的，几乎全长cDNA序列编码TLP 4，TLP 6，TLP 7，和TLP 8。cDNA的特征在于具有非常高GC含量的序列位点，并且它们编码具有171-233个氨基酸残基的蛋白质。推测的蛋白质的N-末端之前的22-25个氨基酸残基的推定的信号肽。用Clustal W的树状图将相似的序列分配给我们在这项工作中获得的序列。
Abstract Eight thaumatin-like proteins of barley (Hordeum vulgare L.) (TLP1–TLP8) were detected in Drechslera teres infected leaves by two-dimensional electrophoresis followed by N-terminal microsequencing: four of them are acidic; four are basic proteins. Partial amino acid sequence data were used to generate polymerase chain reaction (PCR) clones employed for the isolation of four novel, nearly full-length cDNA sequences encoding TLP4, TLP6, TLP 7, and TLP 8. The cDNAs are characterized by sequence sites of very high GC content and they encode proteins with 171–233 amino acid residues. The N-termini of the deduced proteins were preceded by putative signal peptides of 22–25 amino acid residues. With a Clustal W based dendrogram similar sequences were assigned to those we obtained in this work.