Biomolecular stiffness detection based on positive frequency shift of CMOS compatible gigahertz solidly mounted resonators.
Biomolecular stiffness detection based on positive frequency shift of CMOS compatible gigahertz solidly mounted resonators.
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DOI：
10.1016/j.bios.2017.05.002
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发表时间：
2017-10
影响因子：
12.6
通讯作者：
Qingrui Yang;S. Pan;Yuan Zhao;Hao F. Zhang;W. Pang;X. Duan
中科院分区：
文献类型：
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作者：
Qingrui Yang;S. Pan;Yuan Zhao;Hao F. Zhang;W. Pang;X. Duan
在这项工作中，吉赫固体安装谐振器（SMR）（2.5 GHz）的设计和制造，构建一个新的粒子谐振器系统，实现生物分子刚度传感的真实的时间。系统的正频移被用来估计连接在SMR和附着颗粒之间的生物分子的刚度。通过对系统的一般块-弹簧模型的数学分析，揭示了系统的工作原理。从声共振模态的角度，用有限元方法对这种弹性相互作用的机理作了进一步的解释。以生物素-链霉亲和素、抗体和抗原结合系统为模型分子连接体，研究了不同粒径和密度下的频率偏移。通过调节与颗粒连接的抗原的浓度来实现不同的接头刚度，所述颗粒与固定在SMR上的抗体形成特异性结合。实验结果与仿真结果吻合较好，表明粒子谐振系统是实现生物分子刚度实时检测的有效方法。
In this work, gigahertz solidly mounted resonators (SMRs) (2.5 GHz) were designed and fabricated to construct a novel particle-resonator system to achieve the biomolecular stiffness sensing in real time. The positive frequency shift of the system was used to estimate the stiffness of biomolecules connecting between the SMR and attached particles. The working principle was revealed by the mathematical analysis of the general block-spring model of the system. Further interpretations about the mechanism of such elastic interaction from the perspective of acoustic resonant modes of SMRs were given by finite element method. Biotin-streptavidin, antibody and antigen binding system were used as model molecular linkers to study the frequency shift varied with different particle diameters and particle densities. Different linker stiffness was realized by adjusting the concentrations of antigens connected with particles which form specific binding with antibodies immobilized on the SMR. The results fairly agree with the simulation results demonstrating the proposed particle-resonator system as an effective method to realize the real-time biomolecular stiffness detection.