Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing.
Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing.
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DOI：
10.1016/j.jhazmat.2015.04.010
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发表时间：
2015-09
影响因子：
13.6
通讯作者：
Jiemin Zhou;Xuemei Zhou;Yuguang Li;J. Xing
中科院分区：
文献类型：
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作者：
Jiemin Zhou;Xuemei Zhou;Yuguang Li;J. Xing
生物法处理烟气是一种有效的烟气处理方法，但存在硫化物抑制现象。然而，没有硫化物的毒性效应，在haloalkaliphilic生物反应器中观察到。乙醇进料生物反应器的性能分别优于乳酸、葡萄糖和甲酸进料生物反应器。为了有力地支持这一结果，应用Illumina MiSeq 16S rRNA基因的配对末端测序来研究细菌群落。共获得389，971个有效序列，所有序列均归属于10，220个操作分类单位（OTU），相似性为97%。在葡萄糖喂养的生物反应器中的细菌群落表现出最大的丰富性和均匀性。硫酸盐还原菌（SRB）的相对丰度最高的乙醇流生物反应器中发现，这可以解释为什么乙醇流生物反应器的性能是最好的。同时检测到不同类型的硫酸盐还原菌、硫氧化菌和硫还原菌，表明硫可能在这些微生物之间进行循环。由于高通量16S rRNA基因双末端测序技术提高了细菌群落分析的分辨率，因此检测到了许多稀有微生物，如Halanaerobium、Halothiobacillus、Desulfonatronum、Syntrophobacteria和Fusibacter。对这些细菌的16S rRNA基因测序将提供更多关于细菌群落的功能和系统发育信息。
Biological technology used to treat flue gas is useful to replace conventional treatment, but there is sulfide inhibition. However, no sulfide toxicity effect was observed in haloalkaliphilic bioreactors. The performance of the ethanol-fed bioreactor was better than that of lactate-, glucose-, and formate-fed bioreactor, respectively. To support this result strongly, Illumina MiSeq paired-end sequencing of 16S rRNA gene was applied to investigate the bacterial communities. A total of 389,971 effective sequences were obtained and all of them were assigned to 10,220 operational taxonomic units (OTUs) at a 97% similarity. Bacterial communities in the glucose-fed bioreactor showed the greatest richness and evenness. The highest relative abundance of sulfate-reducing bacteria (SRB) was found in the ethanol-fed bioreactor, which can explain why the performance of the ethanol-fed bioreactor was the best. Different types of SRB, sulfur-oxidizing bacteria, and sulfur-reducing bacteria were detected, indicating that sulfur may be cycled among these microorganisms. Because high-throughput 16S rRNA gene paired-end sequencing has improved resolution of bacterial community analysis, many rare microorganisms were detected, such asHalanaerobium,Halothiobacillus,Desulfonatronum,Syntrophobacter, andFusibacter. 16S rRNA gene sequencing of these bacteria would provide more functional and phylogenetic information about the bacterial communities.