Chloroplast TatC plays a direct role in thylakoid ΔpH-dependent protein transport
Chloroplast TatC plays a direct role in thylakoid ΔpH-dependent protein transport
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DOI：
10.1016/s0014-5793(01)02626-6
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发表时间：
2001-07-13
期刊：
影响因子：
3.5
通讯作者：
Cline, K
中科院分区：
文献类型：
--
作者：
Mori, H;Summer, EJ;Cline, K
类囊体δ pH依赖性途径转运折叠蛋白。鉴定的成分包括Hcf 106和Tha 4。这些蛋白质的直向同源物加上称为TatC的膜蛋白是同源细菌达特系统所必需的。cpTatC是一种完整的类囊体膜蛋白，通过体外叶绿体输入和免疫印迹确定，cpTatC抗体特异性地抑制体外类囊体Delta pH依赖性途径。cpTatC以与估计的易位位点大约相同的量存在，而Hcf 106和Tha 4以5-8倍过量存在。这些结果与该系统的机理模型有关。(C)2001年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
The thylakoid Delta pH-dependent pathway transports folded proteins. Identified components include Hcf106 and Tha4. Orthologs of these proteins plus a membrane protein called TatC are essential for the homologous bacterial Tat system, Here we report identification of a chloroplast TatC (cpTatC). cpTatC is an integral thylakoid membrane protein as determined by in vitro chloroplast import and immunoblotting, Antibody to cpTatC specifically inhibited the thylakoid Delta pH-dependent pathway in vitro. cpTatC is present in about the same quantity as estimated translocation sites, whereas Hcf106 and Tha4 are present in 5-8-fold excess. These results are relevant to mechanistic models for this system. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.