Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice
Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice
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DOI：
10.3390/agriculture12060806
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发表时间：
2022-06
期刊：
影响因子：
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通讯作者：
Jian Wang;Yu-yan Cao;Kejian Wang;Chaolei Liu
中科院分区：
文献类型：
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作者：
Jian Wang;Yu-yan Cao;Kejian Wang;Chaolei Liu
利用杂合种质与mtl单倍体诱导系进行杂交，在活体条件下产生双单倍体（DH）有望改变现代水稻育种。然而，这种技术是有限的，因为单倍体诱导剂和花粉受体具有不同步的抽穗日期。为了解决这一障碍，我们开发了一组多抽穗期mtl单倍体诱导系，其产生花粉超过35天。我们用CRISPR-Cas9系统编辑了杂交水稻中的MTL基因。然后在T1代中筛选出无转基因纯合突变体，并通过单粒下降法繁殖到T4代。我们获得了547个不同抽穗期（73 - 110天）的mtl单倍体诱导物，并选择了16个连续开花的核心群体。核心板的结实率和单倍体诱导率分别为4.0-12.7%和2.8- 12.0%。因此，我们的策略，使用多抽穗期mtl单倍体诱导物可以加速体内DH技术在水稻育种中的应用。
In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl haploid inducer lines promises to transform modern rice (Oryza sativa) breeding. However, this technology is limited, as haploid inducers and pollen acceptors have asynchronous heading dates. To address this obstacle, we developed a panel of multiple-heading-date mtl haploid inducer lines that produce pollen for more than 35 days. We edited the MTL gene in a hybrid rice with the CRISPR-Cas9 system. We then selected transgene-free homozygous mutants in the T1 generation and reproduced to T4 generation by single-seed descent method. We obtained 547 mtl haploid inducers with diverse heading dates (from 73 to 110 days) and selected 16 lines comprising a core population with continuous flowering. The seed-setting rate and haploid induction rate (HIR) of the core panel were 4.0–12.7% and 2.8–12.0%, respectively. Thus, our strategy of using multiple-heading-date mtl haploid inducers could accelerate the use of in vivo DH technology in rice breeding.