Nucleotide sequence analysis of a 21-kbp region of the genome of human herpesvirus-6 containing homologues of human cytomegalovirus major immediate-early and replication genes.
Nucleotide sequence analysis of a 21-kbp region of the genome of human herpesvirus-6 containing homologues of human cytomegalovirus major immediate-early and replication genes.
复制标题
人类疱疹病毒 6 基因组 21 kbp 区域的核苷酸序列分析，该区域包含人类巨细胞病毒主要立即早期基因和复制基因的同源物。
DOI：
10.1006/viro.1994.1589
复制
发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
J. Nicholas
中科院分区：
文献类型：
--
作者：
J. Nicholas
人类疱疹病毒6型(HHV-6)是一种CD4+T淋巴细胞的疱疹病毒，属于β疱疹病毒亚群。该实验室此前已经报道了对HHV-6基因组进行大规模序列分析的结果，并鉴定了推测的即刻早期(IE)调控基因和相关的反式激活功能。在这份报告中，我对HHV-6毒株U1102基因组一个21kbp的区域进行了序列分析，该区域与推测的主要IE(Mie)基因座的近端区域相邻。在该区域(基因组位置109-130kbp)，除了编码可能的病毒DNA复制功能的基因外，还包括人类巨细胞病毒(HCMV)IE2mie基因(UL122)的同源基因。后者包括与HCMV UL102和UL105基因产物相对应的解旋酶-Primase复合体的组成部分，以及在甲型疱疹病毒中保守的起源结合蛋白的同源物。在测序的基因座中也发现了几个HHV-6独有的基因。这些发现将有助于鉴定和确定HHV-6转录调控基因的完整谱系，并将为进一步分析这种贝塔疱疹病毒的分子生物学提供基础。
Human herpesvirus-6 (HHV-6) is a CD4+ T-lymphotropic herpesvirus that is a member of the betaherpesvirus subgroup. This laboratory has reported previously the results of large-scale sequence analyses of the HHV-6 genome and has identified putative immediate-early (IE) regulatory genes and associated transactivation functions. In this report I present the sequence analysis of a 21-kbp region of the genome of HHV-6 strain U1102 that is contiguous with the proximal region of the putative major IE (MIE) locus. Within this region (genomic positions 109-130 kbp) is a homologue of the human cytomegalovirus (HCMV) IE2 MIE gene (UL122), in addition to genes encoding probable virus DNA replication functions. The latter include components of the helicase-primase complex, corresponding to HCMV UL102 and UL105 gene products, and a homologue of the origin binding protein conserved among the alphaherpesviruses. Several genes unique to HHV-6 also have been identified within the sequenced locus. These findings will contribute to efforts to identify and functionally characterize the complete repertoire of HHV-6 transcriptional regulatory genes and will provide a basis for the further analysis of the molecular biology of this betaherpesvirus.