IQGAP1 regulates hyaluronan-mediated fibroblast motility and proliferation
IQGAP1 regulates hyaluronan-mediated fibroblast motility and proliferation
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DOI：
10.1016/j.cellsig.2012.05.013
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发表时间：
2012-09-01
影响因子：
4.8
通讯作者：
Heldin, Paraskevi
中科院分区：
文献类型：
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作者：
Kozlova, Inna;Ruusala, Aino;Heldin, Paraskevi
IQGAP1是一种重要的支架蛋白，与透明质酸受体CD44形成复合体。在这项研究中，我们研究了IQGAP1在透明质酸介导的成纤维细胞迁移和增殖中的重要性。透明质酸诱导F-肌动蛋白纤维的形成和局部粘连，这依赖于IQGAP1。IQGAP1是透明质酸所必需的，但不是血小板衍生生长因子BB诱导的细胞迁移所必需的；IQGAP1是透明质酸和PDGF BB所介导的成纤维细胞增殖所必需的，但不是10%胎牛血清诱导的增殖所必需的。IQGAP1的缺失抑制了透明质酸诱导的rac1激活，增强了RhoA的激活。综上所述，这些发现表明IQGAP1在透明质酸刺激的成纤维细胞迁移和增殖中发挥重要作用。(C)2012 Elsevier Inc.保留所有权利。
IQGAP1, an essential scaffolding protein, forms a complex with the hyaluronan receptor CD44. In this study, we have examined the importance of IQGAP1 for hyaluronan-mediated fibroblast migration and proliferation. Hyaluronan induced formation of F-actin fibers and focal adhesions, which was dependent on IQGAP1. IQGAP1 was required for hyaluronan- but not for platelet-derived growth factor (PDGF)-BB-induced cell migration, and was required for both hyaluronan- and PDGF-BB-mediated fibroblast proliferation, but not for proliferation induced by 10% fetal bovine serum. Depletion of IQGAP1 suppressed hyaluronan-induced activation of Rac1 and enhanced the activation of RhoA. Taken together, these findings indicate important roles for IQGAP1 in hyaluronan-stimulated migration and proliferation of fibroblasts. (C) 2012 Elsevier Inc. All rights reserved.