RTE1 is a golgi-associated and ETR1-dependent negative regulator of ethylene responses
RTE1 is a golgi-associated and ETR1-dependent negative regulator of ethylene responses
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RTE1 是乙烯反应的高尔基体相关且 ETR1 依赖性负调节因子
DOI：
10.1104/pp.107.104299
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发表时间：
2007-09-01
期刊：
影响因子：
7.4
通讯作者：
Wen, Chi-Kuang
中科院分区：
文献类型：
--
作者：
Zhou, Xin;Liu, Qian;Wen, Chi-Kuang
拟南芥 (Arabidopsis thaliana) RTE1 编码膜蛋白并负向调节乙烯反应。遗传和转化研究表明，野生型 RTE1 的功能主要依赖于 ETR1，并且可以独立于其他受体。 RTE1 过度表达引起的乙烯不敏感性很大程度上被 etr1-7 突变所掩盖，但不会被任何其他受体突变所掩盖。野生型ETR1 N 末端足以激活RTE1 功能，并且etr1(1-349) 的异位表达恢复了etr1-7 中35S::RTE1 赋予的乙烯不敏感性。 RTE1 N 末端对于 etr1-2 功能并不是必需的，rte1(N Delta 49) 的表达（N 末端删除了 49 个氨基酸残基）恢复了 etr1-2 rte1-2 中的乙烯不敏感性。绿色荧光蛋白 (GFP)-RTE1 的异位表达赋予野生型乙烯不敏感性，并且 GFP 融合体在细胞质内显示出快速运动。 GFP-RTE1 和 EYFP-NAG 蛋白共定位，并且 Brefeldin A 处理引起 GFP-RTE1 聚集，表明 RTE1 是一种高尔基体相关蛋白。我们的结果表明 RTE1 功能对 ETR1 具有特异性，并且内膜可能在乙烯信号转导中发挥作用。
Arabidopsis (Arabidopsis thaliana) RTE1 encodes a membrane protein and negatively regulates ethylene responses. Genetic and transformation studies suggest that the function of the wild-type RTE1 is primarily dependent on ETR1 and can be independent on the other receptors. Ethylene insensitivity caused by the overexpression of RTE1 is largely masked by the etr1-7 mutation, but not by any other receptor mutations. The wild-type ETR1 N terminus is sufficient to the activation of the RTE1 function and the ectopic expression of etr1(1-349) restored ethylene insensitivity conferred by 35S::RTE1 in etr1-7. The RTE1 N terminus is not essential to the etr1-2 function and the expression of rte1(N Delta 49), which has an N-terminal deletion of 49 amino acid residues, restored ethylene insensitivity in etr1-2 rte1-2. The ectopic expression of GREEN FLUORESCENT PROTEIN (GFP)-RTE1 conferred ethylene insensitivity in wild type and the GFP fusion displayed fast movement within the cytoplasm. The GFP-RTE1 and EYFP-NAG proteins colocalized and the Brefeldin A treatment caused aggregation of GFP-RTE1, suggesting RTE1 is a Golgi-associated protein. Our results suggest specificity of the RTE1 function to ETR1 and that endomembranes may play a role in the ethylene signal transduction.