Cytoplasmic gamma actin as a Z-disc protein.
Cytoplasmic gamma actin as a Z-disc protein.
复制标题
细胞质伽马肌动蛋白作为 Z 盘蛋白。
DOI：
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发表时间：
2001
影响因子：
3.1
通讯作者：
H. Yorifuji
中科院分区：
文献类型：
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作者：
T. Nakata;Y. Nishina;H. Yorifuji
为了研究骨骼肌中细胞质γ肌动蛋白的精确定位及其与肌营养不良蛋白分子的关系，我们设计了一种针对细胞质γ肌动蛋白N-末端肽的抗体。使用SDS-PAGE和等电聚焦（IEF）凝胶的Western印迹分析显示，该抗体仅与具有γ运动性的肌动蛋白亚型反应，证实该抗体对细胞质（非肌肉）γ肌动蛋白具有特异性。成年小鼠骨骼肌的免疫组织化学分析显示，抗体在横截面中呈点状染色模式，在纵截面中呈条纹状染色模式。双重免疫染色技术揭示了细胞质γ肌动蛋白与α-辅肌动蛋白的共定位，这意味着Z-盘上的肌动蛋白的本地化。与以前的发现（1）相反，我们没有检测到细胞色素氧化酶（线粒体标记物）与这种肌动蛋白的共定位。
To investigate the precise localization of cytoplasmic gamma actin in skeletal muscle and the relationship to dystrophin molecules, we designed an antibody against the N-terminal peptide of cytoplasmic gamma actin. Western blot analysis using SDS-PAGE and isoelectric focusing (IEF) gel revealed that the antibody reacted only with the actin isoforms having gamma motility, confirming that the antibody is specific to the cytoplasmic (nonmuscle) gamma actin. Immunohistochemical analysis of the skeletal muscle of the adult mouse revealed a dot-like staining pattern of the antibody in transverse sections and a striated staining pattern in longitudinal sections. The double immunostaining technique revealed the colocalization of cytoplasmic gamma actin with alpha-actinin, implying the localization of the actin on the Z-disc. Contrary to previous findings (1), we did not detect the colocalization of cytochrome oxidase, a mitochondria marker, with this actin.