Dual-functional beta-CD@CdS nanorod/WS2 nanosheet heterostructures coupled with strand displacement reaction-mediated photocurrent quenching for an ultrasensitive MicroRNA-21 assay
Dual-functional beta-CD@CdS nanorod/WS2 nanosheet heterostructures coupled with strand displacement reaction-mediated photocurrent quenching for an ultrasensitive MicroRNA-21 assay
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双功能β-CD@CdS纳米棒/WS2纳米片异质结构与链置换反应介导的光电流猝灭相结合，用于超灵敏的MicroRNA-21测定
DOI：
10.1016/j.electacta.2019.135581
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发表时间：
2020
影响因子：
6.6
通讯作者：
Xue Huaiguo
中科院分区：
文献类型：
--
作者：
Zang Yang;Fan Jing;Zhang Hang;Xu Qin;Jiang Jingjing;Xue Huaiguo
In this work, we first introduced beta-cyclodextrin-functionalized CdS nanorod (β-CD@CdS NR)/WS2nanosheet (NS) heterostructures as dual-functional building blocks for designing an ultrasensitive photoelectrochemical bioassay of microRNA-21 based on cyclic strand displacement reaction (SDR)-mediated Cu2+quenching and host-guest interaction between β-CD and adamantane (ADA), in which β-CD@CdS NR/WS2NS heterojunction serves as a signaling and linking unit, and avidin-labeled CuO (Avidin-CuO) nanoparticle as a quenching unit. In the presence of microRNA-21, ADA-labeled hairpin DNA1 was unfolded by DNA hybridization and triggered SDR cycling with the aid of biotin-labeled hairpin DNA2, which generated plenty of double-stranded DNA structures with biotin molecules at the outer terminal point of dsDNA. When Avidin-CuO was immobilized onto the electrode surface via biotin-avidin affinity binding and then dissolved by hydrochloric acid, the released Cu2+could react with the CdS NR to generate CuxS, leading to a decreased photocurrent response. Under the optimized conditions, this biosensor displayed desirable linearity in the range from 0.1 fM to 10 pM, a low detection limit of 25.1 aM and satisfactory selectivity, and was applied to microRNA-21 analysis in serum samples, holding great potential for early diagnosis of some cancers.
