Systemic Induction and Role of Mitochondrial Alternative Oxidase and Nitric Oxide in a Compatible Tomato-Tobacco mosaic virus Interaction
Systemic Induction and Role of Mitochondrial Alternative Oxidase and Nitric Oxide in a Compatible Tomato-Tobacco mosaic virus Interaction
复制标题
线粒体替代氧化酶和一氧化氮在相容性番茄-烟草花叶病毒相互作用中的系统诱导和作用
DOI：
10.1094/mpmi-23-1-0039
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发表时间：
2010-01-01
影响因子：
3.5
通讯作者：
Yu, Jing-Quan
中科院分区：
文献类型：
--
作者：
Fu, Li-Jun;Shi, Kai;Yu, Jing-Quan
研究了线粒体交替氧化酶(AOX)在病毒诱导番茄对烟草花叶病毒(TMV)系统防御中的作用及其与一氧化氮(NO)的关系。接种TMV后0.5d，上部未接种TMV叶片中NO合成和AOX活性迅速增加。用非致死浓度的外源氰化钾(KCN)和NO供体二乙胺NONOate(DEA/NO)处理未接种的上部叶片，可显著诱导AOX转录本的积累，减少TMV病毒RNA的积累，提高光系统II下叶片的光化学量子产率。NO清除剂的预处理几乎完全阻断了TMV诱导的AOX诱导，显著增加了TMV的感受性。AOX抑制剂水杨氧肟酸(SHAM)处理降低了DEA/NO诱导的抗氰化物呼吸，并部分抑制了诱导的TMV抗性。相反，KCN和Sham预处理对NO的产生影响很小，NO清除剂预处理不影响KCN诱导的AOX诱导和对TMV的抗性。这些结果表明，TMV诱导的NO产生在上游作用，并介导AOX诱导，AOX诱导继而诱导线粒体选择性电子传递，并触发对病毒病原体的系统基础防御。
The role of mitochondrial alternative oxidase (AOX) and the relationship between AOX and nitric oxide (NO) in virus-induced systemic defense to Tobacco mosaic virus (TMV) were investigated in susceptible tomato (Solanum lycopersicum) plants. TMV inoculation to the lower leaves induced a rapid NO synthesis and AOX activation in upper uninoculated leaves as early as 0.5 day postinoculation. Application of exogenous potassium cyanide (KCN, a cytochrome pathway inhibitor) at nonlethal concentrations and NO donor diethylamine NONOate (DEA/NO) to the upper uninoculated leaves greatly induced accumulation of AOX transcript, reduced TMV viral RNA accumulation, and increased the leaf photochemical quantum yield at photosystem II. Pretreatment with NO scavenger almost completely blocked TMV-induced AOX induction and substantially increased TMV susceptibility. Salicylhydroxamic acid (SHAM, an AOX inhibitor) pretreatment reduced the DEA/NO-induced cyanide-resistant respiration and partially compromised induced resistance to TMV. Conversely, KCN and SHAM pretreatment had very little effect on generation of NO, and pretreatment with NO scavenger did not affect KCN-induced AOX induction and TMV resistance. These results suggest that TMV-induced NO generation acts upstream and mediates AOX induction which, in turn, induces mitochondrial alternative electron transport and triggers systemic basal defense against the viral pathogen.