Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy
Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy
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DOI：
10.1073/pnas.0813327106
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发表时间：
2009-08-11
影响因子：
11.1
通讯作者：
Gambhir, Sanjiv S.
中科院分区：
文献类型：
--
作者：
Zavaleta, Cristina L.;Smith, Bryan R.;Gambhir, Sanjiv S.
拉曼光谱是一种新开发的非侵入性临床前成像技术，为分子成像领域提供了皮摩尔级的灵敏度和多路复用能力。在这项研究中，我们证明了拉曼光谱的能力，分离多达10种不同类型的表面增强拉曼散射（Sers）纳米粒子在一个活的小鼠后，s.c.注射基于这些光谱结果，我们同时静脉注射了五种最强烈和光谱独特的Sers纳米粒子，以成像它们在肝脏中的自然积累。所有五种类型的Sers纳米粒子成功地识别和光谱分离使用我们优化的非侵入性拉曼成像系统。此外，我们能够线性相关的拉曼信号与Sers浓度后，注入四个光谱独特的Sers纳米粒子或s.c. (R-2= 0.998）或i. v.（R-2 = 0.992）。这些结果显示了在活体受试者中进行多重成像的巨大潜力，在这种情况下，几种靶向Sers探针可以更好地检测与特定疾病相关的多种生物标志物。
Raman spectroscopy is a newly developed, noninvasive preclinical imaging technique that offers picomolar sensitivity and multiplexing capabilities to the field of molecular imaging. In this study, we demonstrate the ability of Raman spectroscopy to separate the spectral fingerprints of up to 10 different types of surface enhanced Raman scattering (SERS) nanoparticles in a living mouse after s.c. injection. Based on these spectral results, we simultaneously injected the five most intense and spectrally unique SERS nanoparticles i.v. to image their natural accumulation in the liver. All five types of SERS nanoparticles were successfully identified and spectrally separated using our optimized noninvasive Raman imaging system. In addition, we were able to linearly correlate Raman signal with SERS concentration after injecting four spectrally unique SERS nanoparticles either s.c. (R-2 = 0.998) or i.v. (R-2 = 0.992). These results show great potential for multiplexed imaging in living subjects in cases in which several targeted SERS probes could offer better detection of multiple biomarkers associated with a specific disease.