Isolation and characterization of a peroxidase from the airway
Isolation and characterization of a peroxidase from the airway
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DOI：
10.1165/ajrcmb.17.1.2719
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发表时间：
1997-07-01
影响因子：
6.4
通讯作者：
Conner, GE
中科院分区：
文献类型：
--
作者：
Salathe, M;Holderby, M;Conner, GE
绵羊气道粘液能有效地清除过氧化氢，这是气道炎症的重要介质。在这里，清除活性被鉴定为由气道上皮的杯状细胞产生并分泌到气道腔中的过氧化物酶。绵羊气道过氧化物酶活性通过两步从气道灌洗液中纯化，使用阳离子交换和凝集素亲和层析，产生明显均一的82-kD糖蛋白，绵羊气道过氧化物酶占气道粘液中总蛋白的约1%，因此是气道分泌物中丰富的酶。纯化的过氧化物酶的吸收光谱在412 nm处显示出指示血红素蛋白的主峰。纯化酶的A（412）/A（280）比值为0.86。绵羊气道过氧化物酶的吸收光谱、氧化卤化物的能力、对抑制剂的敏感性和在十二烷基硫酸钠凝胶上的表观分子量表明，气道过氧化物酶与乳过氧化物酶相似，但与髓过氧化物酶、嗜酸性粒细胞过氧化物酶和谷胱甘肽过氧化物酶不同。绵羊呼吸道过氧化物酶是一种新分离的和丰富的呼吸道粘液酶，其可用于控制AH-途径中的活性氧物质，并通过催化杀生物化合物的形成来预防感染。
Sheep airway mucus can potently scavenge hydrogen peroxide, an important mediator of airway inflammation, Here, the scavenging activity was identified as a peroxidase produced by goblet cells of the airway epithelium and secreted into the ah-way lumen, Ovine airway peroxidase activity was purified similar to 100-fold from airway lavage fluid in two steps, using cation exchange and lectin affinity chromatography, yielding an apparently homogeneous 82-kD glycoprotein, Ovine airway peroxidase represented about 1% of the total protein in ah-way mucus and thus was an abundant enzyme in airway secretions. The absorbance spectrum of the purified peroxidase showed a major peak at 412 nm indicative of a hemoprotein. The ratio of A(412)/A(280) of the purified enzyme was 0.86, The absorption spectrum of ovine airway peroxidase, its ability to oxidize halides, its sensitivity to inhibitors and its apparent molecular mass on sodium dodecyl sulfate gels showed that airway peroxidase was similar to lactoperoxidase but distinguished from myeloperoxidase, eosinophil peroxidase as well as from glutathione peroxidases, Based on these observations, ovine airway peroxidase is a newly isolated and abundant enzyme of airway mucus which may function to control reactive oxygen species in the ah-way and to prevent infection by catalyzing the formation of biocidal compounds.