INSULIN BIOSYNTHESIS - EVIDENCE FOR A PRECURSOR
INSULIN BIOSYNTHESIS - EVIDENCE FOR A PRECURSOR
复制标题
DOI：
10.1126/science.157.3789.697
复制
发表时间：
1967-01-01
期刊：
影响因子：
56.9
通讯作者：
ATEN, B
中科院分区：
文献类型：
--
作者：
STEINER, DF;CUNNINGHAM, D;ATEN, B
人胰岛细胞肿瘤组织和大鼠分离的朗格汉斯胰岛在体外将放射性氨基酸掺入胰岛素和更大的酸醇可溶性蛋白质中，该蛋白质可以通过凝胶过滤与胰岛素分离。氨基酸比胰岛素更早地融入到较大的蛋白质中；仅在胰岛孵育约 30 分钟后。胰岛素中是否开始出现放射性？在没有新的肽键合成（环己酰亚胺）的情况下，或在与未标记的氨基酸（chase）一起孵育的情况下，证明了较大蛋白质的约 70％ 的放射性转移到了胰岛素。结果表明较大的蛋白质是胰岛素生物合成的前体。建议将该蛋白质命名为“胰岛素原”。
Human islet cell tumor tissue and isolated islets of Langerhans from rats incorporated radioactive amino acids in vitro into insulin and a larger acid-alcohol soluble protein which could be separated from insulin by gel filtration. The amino acids were incorporated into the larger protein earlier than into insulin; only after incubation of islets for approximately 30 min. did radioactivity begin to appear in insulin. The transfer of about 70% of the radioactivity of the larger protein to insulin was demonstrated in the absence of new peptide bond synthesis (cyclo-heximide), or during incubation with unlabeled amino acid (chase). The results indicate that the larger protein is a precursor in the biosynthesis of insulin. The name "proinsulin" is suggested for this protein.