Biochemical and histochemical traits: a promising way to screen resistance against spot blotch (Bipolaris sorokiniana) of wheat
Biochemical and histochemical traits: a promising way to screen resistance against spot blotch (Bipolaris sorokiniana) of wheat
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DOI：
10.1007/s10658-013-0290-8
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发表时间：
2013-12-01
影响因子：
1.8
通讯作者：
Joshi, Arun K.
中科院分区：
文献类型：
--
作者：
Eisa, Musaed;Chand, Ramesh;Joshi, Arun K.
小麦斑点病(Bipolaris Sorokiniana)是世界上温暖潮湿地区小麦的一种严重病害。以感病亲本Sonalika和抗病亲本扬麦6号为材料，研究了病程曲线下面积(AUDPC)、病情严重程度(DS)和病斑大小3个致病成分，以及总酚含量(TPC)、叶绿素含量(CHC)、苯丙氨酸解氨酶(PAL)活性和木质素沉积4个生化组织化学因子。其目的是确定一种或多种稳健可靠的抗性工具，无论是物理、生化还是组织化学的，以便于对斑点病的选择。感病亲本和重组自交系的DS、AUDPC和病变大小均高于抗病亲本。接种后48h，抗性最强的重组自交系的平均TPC(246mgg(-1)鲜重)显著高于最敏感的(181.5 mg没食子酸g(-1)鲜重)。抗性品系和感病品系的平均PAL分别为928.4~96.0mmol.肉桂酸mg(-1)，48HAI鲜重。同样，抗性重组自交系的木质素沉积显著高于敏感重组自交系。生化和组织化学参数与抗性显著相关，对于育种材料的筛选和提高斑点病表型的精确度具有很强的实用性。
Spot blotch caused by Bipolaris sorokiniana is a serious disease of wheat in warmer and humid regions of the world. Three blighting components, area under disease progress curve (AUDPC), disease severity (DS) and lesion size along with four biochemical and histochemical factors viz., total phenol content (TPC), chlorophyll content (CHC), phenylalanine ammonia-lyase (PAL) activity and lignin deposition were studied in a recombinant inbred lines (RILs) population involving parents "Sonalika" (susceptible) and "Yangmai 6" (resistant). The objective was to identify one or more robust and reliable tools of resistance, physical, biochemical or histochemical, to facilitate selection against spot blotch. The DS, AUDPC and lesion size were higher in the susceptible parent and RILs compared to the resistant. The mean TPC (246 mg Gallic acid g(-1) fresh weight) of the most resistant RIL was significantly higher than the most susceptible (181.5 mg Gallic acid g(-1) fresh weight) at 48 h after inoculation (hai). The mean SPAD value for CHC varied between 48.8 in the most resistant RILs to 8.8 in the most susceptible, while the mean PAL varied between 928.4 and 96.0 mu moles Cinnamic acid mg(-1) fresh weight at 48 hai in resistant and susceptible RILs, respectively. Likewise, lignin deposition was significantly higher in resistant RILs compared to the susceptible. The biochemical and histochemical parameters were significantly correlated with resistance and appeared robust for facilitating screening of breeding material and for increased precision in phenotyping against spot blotch.