Mass spectrometry: come of age for structural and dynamical biology.
Mass spectrometry: come of age for structural and dynamical biology.
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DOI：
10.1016/j.sbi.2011.08.002
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发表时间：
2011-10
影响因子：
6.8
通讯作者：
Ruotolo BT
中科院分区：
文献类型：
--
作者：
Benesch JL;Ruotolo BT
在过去的二十年里，质谱（MS）已经成为结构生物学的真正方法。MS可以告知蛋白质组织的所有水平，并能够对其内在动力学进行定量评估。MS的主要优点是它是一种灵敏的、高分辨率的分离技术，具有广泛的适用性，从而允许在复杂混合物的背景下询问瞬时蛋白质组装体。在这里，我们描述了分子水平的信息是如何从MS实验，以及它如何可以与空间和动力学的限制从其他结构生物学方法，允许混合研究蛋白质的结构和运动。
Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique with wide applicability, and thereby allows the interrogation of transient protein assemblies in the context of complex mixtures. Here we describe how molecular-level information is derived from MS experiments, and how it can be combined with spatial and dynamical restraints obtained from other structural biology approaches to allow hybrid studies of protein architecture and movements.