The histopathology of experimental trachoma: ultrastructural changes in the conjunctival epithelium.
The histopathology of experimental trachoma: ultrastructural changes in the conjunctival epithelium.
复制标题
实验性沙眼的组织病理学：结膜上皮的超微结构变化。
DOI：
10.1093/infdis/153.5.870
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Taylor,HR
中科院分区：
文献类型：
--
作者：
Patton,DL;Taylor,HR
用眼接种沙眼衣原体血清B型引起食蟹猴实验性急性结膜炎。通过光镜、透射电镜和扫描电镜观察细胞对衣原体结膜炎感染的反应。一次接种引起的自限性急性结膜炎。结膜内可见中度淋巴细胞浸润。反复接种后，出现慢性结膜炎（沙眼）。在这些组织中有明显的淋巴滤泡和生发中心。扫描电镜显示斑块状的细胞改变和微绒毛的缺失。细胞内损伤，通过透射电镜记录，包括细胞质膜破坏和细胞器破裂。炎性浸润包括浆细胞、多形核细胞、淋巴细胞、嗜酸性粒细胞和脱颗粒肥大细胞。单克隆抗体泛白细胞、巨噬细胞和B细胞的免疫过氧化物酶染色表征了单次和多次衣原体感染后的免疫反应。卵泡中心由B细胞组成，帽区有T细胞。生发中心也发现大量巨噬细胞。衣原体感染后结膜组织病理和免疫改变的细胞机制需要进一步研究。
Experimental acute conjunctivitis was produced in cynomolgus monkeys by ocular inoculation with serovar B ofChlamydia trachomatis. The cellular responses to chlamydial conjunctivitis infection were examined by light, transmission, and scanning electron microscopy. A self-limited, acute conjunctivitis resulted from a single primary inoculation. A moderate lymphocytic infiltrate was present in the conjunctiva. After repeated inoculation, a chronic conjunctivitis (trachoma) developed. Prominent lymphoid follicles with distinct germinal centers were present in these tissues. Scanning electron microscopy revealed patchy areas of cellular alteration and loss of microvilli. Intracellular injury, documented by transmission electron microscopy, included disruption of the plasmalemmal membranes and rupture of the cytoplasmic organelles. The inflammatory infiltrate consisted of plasma cells, polymorphonuclear cells, lymphocytes, eosinophils, and degranulating mast cells. The immune response following single and repeated chlamydial infections was characterized by immunoperoxidase staining with monoclonal antibodies to pan-leukocytes, macrophages, and B cells. The center of the follicle was comprised of B cells, with T cells in the cap region. Large macrophages were also found in the germinal center. Further study is required to determine which cellular mechanisms are involved in the histopathologic and immunologic alterations induced in the conjunctiva after chlamydial infection.