Adaptation of a ladder beam walking task to assess locomotor recovery in mice following spinal cord injury
Adaptation of a ladder beam walking task to assess locomotor recovery in mice following spinal cord injury
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DOI：
10.1016/j.bbr.2006.11.042
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发表时间：
2007-02-27
影响因子：
2.7
通讯作者：
Anderson, Aileen J.
中科院分区：
文献类型：
--
作者：
Cummings, Brian J.;Engesser-Cesar, Christie;Anderson, Aileen J.
脊髓损伤（SCI）后的运动障碍通常使用开放领域评定量表进行评估。这些任务的优点是跨越从完全瘫痪到正常行走的范围;然而，它们在特定的恢复水平上缺乏敏感性。此外，大多数补充评估是在大鼠而不是小鼠中开发的。例如，水平梯梁已被用于测量SCI后大鼠的恢复。这个参数任务的结果是一个录像的档案记录的事件，很容易管理，并明确得分。虽然小鼠的阶梯梁装置是可用的，其用于评估SCI小鼠的恢复是罕见的，可能是因为未受伤的小鼠和受伤的小鼠表现出的步骤错位的类型的规范性数据是缺乏的。我们报告了一种改进的阶梯梁仪器和评分系统的发展，以衡量后肢恢复脊椎T9挫伤脊髓损伤小鼠。小鼠梯形梁允许使用标准参数统计检验来评估运动恢复。四个品系小鼠的梯形梁表现是一致的，没有性别差异，观察者之间的评分员间可靠性很高。阶梯梁评分与损伤严重程度成比例，并且可以用于容易地将能够负重站立的小鼠与具有一致的前肢-后肢协调的小鼠分开。重要的是，水平梯梁测试区分在旷场测试中就步进频率而言得分相同的小鼠。(c)2006 Elsevier B. V.保留所有权利。
Locomotor impairments after spinal cord injury (SCI) are often assessed using open-field rating scales. These tasks have the advantage of spanning the range from complete paralysis to normal walking; however, they lack sensitivity at specific levels of recovery. Additionally, most supplemental assessments were developed in rats, not mice. For example, the horizontal ladder beam has been used to measure recovery in the rat after SCI. This parametric task results in a videotaped archival record of the event, is easily administered, and is unambiguously scored. Although a ladder beam apparatus for mice is available, its use in the assessment of recovery in SCI mice is rare, possibly because normative data for uninjured mice and the type of step misplacements injured mice exhibit is lacking. We report the development of a modified ladder beam instrument and scoring system to measure hindlimb recovery in vertebral T9 contusion spinal cord injured mice. The mouse ladder beam allows for the use of standard parametric statistical tests to assess locomotor recovery. Ladder beam performance is consistent across four strains of mice, there are no sex differences, and inter-rater reliability between observers is high. The ladder beam score is proportional to injury severity and can be used to easily separate mice capable of weight-supported stance up to mice with consistent forelimb to hindlimb coordination. Critically, horizontal ladder beam testing discriminates between mice that score identically in terms of stepping frequency in open-field testing. (c) 2006 Elsevier B.V. All rights reserved.