The transcription factor WRKY32 affects tomato fruit colour by regulating YELLOW FRUITED-TOMATO 1, a core component of ethylene signal transduction
The transcription factor WRKY32 affects tomato fruit colour by regulating YELLOW FRUITED-TOMATO 1, a core component of ethylene signal transduction
复制标题
转录因子WRKY32通过调节乙烯信号转导的核心成分YELLOW FRUITED-TOMATO 1影响番茄果实颜色
DOI：
10.1093/jxb/erab113
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发表时间：
2021-03-27
影响因子：
6.9
通讯作者：
Zhao, Lingxia
中科院分区：
文献类型：
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作者：
Zhao, Weihua;Li, Yuhang;Zhao, Lingxia
大多数肉质水果作物的果实品质从根本上与成熟相关的性状有关，包括颜色的变化。在许多更年期水果中，包括番茄（Solanum lycopersicum），植物激素乙烯在调节成熟方面发挥着关键作用。先前基于图谱的 YELLOW FRUITED-TOMATO 1 (YFT1) 克隆表明，它编码 EIN2 蛋白，这是乙烯信号转导的核心成分。发现具有遗传损伤的 YFT1 等位基因在 yft1 番茄突变体中下调，该突变体具有黄色果实表型和扰动的乙烯信号传导。基于生物信息学分析、酵母一杂交分析和电泳迁移率变动分析，我们报告转录因子 WRKY32 调节番茄果实颜色的形成。 WRKY32 与 YFT1 启动子调控区的 W 盒和 W 盒样基序结合并诱导其表达。在WRKY32-RNA1生成品系的番茄果实中，乙烯信号传导减弱，导致乙烯释放抑制、有色体发育延迟、类胡萝卜素积累减少和黄色果实表型。这些结果为通过乙烯信号转导控制番茄果实颜色形成的调控网络提供了新的见解。
Fruit quality in most fleshy fruit crops is fundamentally linked to ripening-associated traits, including changes in colour. In many climacteric fruits, including tomato (Solanum lycopersicum), the phytohormone ethylene plays a key role in regulating ripening. Previous map-based cloning of YELLOW FRUITED-TOMATO 1 (YFT1) revealed that it encodes the EIN2 protein, a core component in ethylene signal transduction. A YFT1 allele with a genetic lesion was found to be down-regulated in the yft1 tomato mutant that has a yellow fruit phenotype and perturbed ethylene signalling. Based on bioinformatic analysis, yeast one hybrid assays and electrophoretic mobility shift assays, we report that transcription factor WRKY32 regulates tomato fruit colour formation. WRKY32 binds to W-box and W-box-like motifs in the regulatory region of the YFT1 promoter and induces its expression. In tomato fruits of WRKY32-RNA1 generated lines, ethylene signalling was reduced, leading to a suppression in ethylene emission, a delay in chromoplast development, decreased carotenoid accumulation, and a yellow fruit phenotype. These results provide new insights into the regulatory networks that govern tomato fruit colour formation via ethylene signal transduction.