Toward a high-resolution Plasmodium falciparum linkage map: Polymorphic markers from hundreds of simple sequence repeats
Toward a high-resolution Plasmodium falciparum linkage map: Polymorphic markers from hundreds of simple sequence repeats
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DOI：
10.1006/geno.1996.0218
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发表时间：
1996-05-01
期刊：
影响因子：
4.4
通讯作者：
Wellems, TE
中科院分区：
文献类型：
--
作者：
Su, XZ;Wellems, TE
从GenBank中的恶性疟原虫序列和基因组DNA文库中的插入片段中共鉴定出507个简单重复序列（SSR或“微卫星”）。合成来自这些SSR中的224个侧翼序列的寡核苷酸引物，并用于PCR测定以测试简单序列长度多态性（SSLP）。在224个SSR中，188个在12个不同的恶性疟原虫株系中显示SSLP;通过物理作图和通过将它们的遗传模式与遗传杂交（HE 3xDd 2）中的限制性片段长度多态性标记的遗传模式进行比较，将这些SSLP中的116个分配到染色体连锁群。恶性疟原虫中主要的SSLP被发现含有[TA]（n）、[T]（n）和[TAA]（n），这一特征使人联想到植物基因组，并与疟疾寄生虫的藻类样起源一致。由于此类SSLP丰富且易于分离，因此它们是恶性疟原虫遗传分析的有力资源。(C)出版社：Academic Press，Inc.
A total of 507 simple sequence repeats (SSRs or ''microsatellites'') were identified from Plasmodium falciparum sequences in GenBank and from inserts in a genomic DNA library. Oligonucleotide primers from sequences that flank 224 of these SSRs were synthesized and used in PCR assays to test for simple sequence length polymorphisms (SSLPs). Of the 224 SSRs, 188 showed SSLPs among 12 different P. falciparum lines; 116 of these SSLPs were assigned to chromosome Linkage groups by physical mapping and by comparing their inheritance patterns against those of restriction fragment length polymorphism markers in a genetic cross (HE3xDd2). The predominant SSLPs in P. falciparum were found to contain [TA](n), [T](n), and [TAA](n), a feature that-is reminiscent of plant genomes and is consistent with the proposed algal-like origin of malaria parasites. Since such SSLPs are abundant and readily isolated, they are a powerful resource for genetic analysis of P. falciparum. (C) 1996 Academic Press, Inc.