Galactosylceramide expression factor-1 induces myogenesis in MDCK and C3H10T1/2 cells
Galactosylceramide expression factor-1 induces myogenesis in MDCK and C3H10T1/2 cells
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DOI：
10.1016/j.abb.2004.02.029
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发表时间：
2004-06-15
影响因子：
3.9
通讯作者：
Tai, T
中科院分区：
文献类型：
--
作者：
Ogura, K;Niino, YS;Tai, T
我们之前报道过半乳糖神经酰胺表达因子-1 （GEF-1）是肝细胞生长因子调节的酪氨酸激酶底物（Hrs/Hgs）的大鼠同源物，可诱导上皮细胞中半乳糖神经酰胺和/或硫脂的表达和形态学改变。在这里，我们发现GEF-1诱导MDCK和C3H10T1/2细胞的肌生成。GEF-1在MDCK细胞中的过表达（MDCK/GEF-1）似乎促进向表达MyoD和肌球蛋白重链（MHC）的成肌细胞的转分化。MDCK/GEF-1细胞还表达了几种对肌肉形成至关重要的dna结合蛋白（MyoD和MEF-2）。这些结果表明GEF-1诱导MDCK细胞进入肌生成的早期阶段。随后，我们测试了GEF-1是否能在C3H10T1/2小鼠成纤维细胞中诱导肌生成，这些成纤维细胞有可能分化为成肌细胞样细胞。事实上，GEF-1诱导的形态学变化与成肌细胞样细胞一致，MyoD和MHC均有表达。结果表明，GEF-1可诱导MDCK和C3H10T1/2细胞向成肌样细胞转化。(C) 2004爱思唯尔公司版权所有。
We previously reported that galactosylceramide expression factor-1 (GEF-1), a rat homolog of hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs/Hgs), induces galactosylceramide and/or sulfatide expression and morphological changes in epithelial cells. Here, we show that GEF-1 induces myogenesis in MDCK and C3H10T1/2 cells. GEF-1 overexpression in MDCK cells (MDCK/GEF-1) appeared to promote trans-differentiation to myoblasts that expressed MyoD and myosin heavy chain (MHC). MDCK/GEF-1 cells also expressed several DNA-binding proteins (MyoD and MEF-2) that are essential for myogenesis. These results suggest that GEF-1 induces MDCK cells to enter an early stage of myogenesis. Subsequently, we tested whether GEF-1 could induce myogenesis in C3H10T1/2 mouse fibroblasts, which have the potential to differentiate into myoblast-like cells. Indeed, GEF-1 induced morphological changes that were consistent with myoblast-like cells, and both MyoD and MHC were expressed. Our results suggest that GEF-1 may induce MDCK and C3H10T1/2 cells to trans-differentiate into myoblast-like cells. (C) 2004 Elsevier Inc. All rights reserved.