A Novel Wheat C-bZIP Gene, TabZIP14-B, Participates in Salt and Freezing Tolerance in Transgenic Plants.
A Novel Wheat C-bZIP Gene, TabZIP14-B, Participates in Salt and Freezing Tolerance in Transgenic Plants.
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新型小麦 C-bZIP 基因 TabZIP14-B 参与转基因植物的耐盐性和耐冻性
DOI：
10.3389/fpls.2017.00710
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发表时间：
2017
影响因子：
5.6
通讯作者：
Kong X
中科院分区：
文献类型：
--
作者：
Zhang L;Zhang L;Xia C;Gao L;Hao C;Zhao G;Jia J;Kong X
C-bZIP类转录因子参与了多种生物学过程，如种子贮藏蛋白（SSP）的产生、对病原菌和非生物胁迫的应答等。然而，我们的知识在小麦C-bZIP基因组的非生物功能仍然有限。在这里，我们提出了一个新的TabZIP 14-B基因在小麦中的功能。该基因属于C-bZIP TF组，包含6个外显子和5个内含子;在四倍体和六倍体小麦的加入中鉴定了3种单倍型。亚细胞定位分析表明，TabZIP 14-B靶向烟草表皮细胞的细胞核。反式激活实验表明TabZIP 14-B具有转录激活能力，能够与酵母中的脱落酸（阿坝）反应元件（ABRE）结合。RT-qPCR结果表明，TabZIP 14-B在根、茎、叶和幼穗中均有表达，且在阿坝、盐、低温和PEG胁迫下表达上调。此外，过量表达TabZIP 14-B的拟南芥植物表现出对盐、冷冻胁迫和阿坝敏感性的增强的耐受性。TabZIP 14-B的过表达导致AtRD 29 A、AtCOR 47、AtRD 20、AtGSTF 6和AtRAB 18基因的表达增加以及几种生理特征的变化。这些结果表明TabZIP 14-B在介导非生物胁迫响应中可以作为正调节剂起作用。
The group C-bZIP transcription factors (TFs) are involved in diverse biological processes, such as the regulation of seed storage protein (SSP) production and the responses to pathogen challenge and abiotic stress. However, our knowledge of the abiotic functions of group C-bZIP genes in wheat remains limited. Here, we present the function of a novel TabZIP14-B gene in wheat. This gene belongs to the group C-bZIP TFs and contains six exons and five introns; three haplotypes were identified among accessions of tetraploid and hexaploid wheat. A subcellular localization analysis indicated that TabZIP14-B was targeted to the nucleus of tobacco epidermal cells. A transactivation assay demonstrated that TabZIP14-B showed transcriptional activation ability and was capable of binding the abscisic acid (ABA) responsive element (ABRE) in yeast. RT-qPCR revealed that TabZIP14-B was expressed in the roots, stems, leaves, and young spikes and was up-regulated by exogenous ABA, salt, low-temperature, and polyethylene glycol (PEG) stress treatments. Furthermore, Arabidopsis plants overexpressing TabZIP14-B exhibited enhanced tolerance to salt, freezing stresses and ABA sensitivity. Overexpression of TabZIP14-B resulted in increased expression of the AtRD29A, AtCOR47, AtRD20, AtGSTF6, and AtRAB18 genes and changes in several physiological characteristics. These results suggest that TabZIP14-B could function as a positive regulator in mediating the abiotic stress response.