Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures
Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures
复制标题
泡状希瓦氏菌 HM13 产生的膜囊泡作为低温分泌生产异源蛋白的前景平台
DOI：
10.1007/978-1-0716-1900-1_12
复制
发表时间：
2021
影响因子：
--
通讯作者：
Kurihara Tatsuo
中科院分区：
文献类型：
--
作者：
Kawamoto Jun;Kurihara Tatsuo
革兰氏阴性菌产生的胞外膜泡(EMVS)可作为疫苗接种平台。在18℃的肉汤中生长，泡状希瓦氏杆菌HM13产生大量的EMV，其中含有一个49 kDa的主要货物蛋白，命名为P49。融合到P49 C末端的增强型绿色荧光蛋白被运送到EMVS，这表明P49可以作为载体将外源蛋白靶向EMVS，以生产具有所需功能的人造EMVS。这种方法对设计的疫苗的制备有潜在的帮助，本章将对此进行详细描述。
Extracellular membrane vesicles (EMVs) produced by Gram-negative bacteria are useful as a vaccine platform. During growth in broth at 18 °C,Shewanella vesiculosaHM13 produces a large number of EMVs that contain a 49-kDa major cargo protein, named P49. Enhanced green fluorescent protein fused to the C-terminus of P49 is delivered to EMVs, suggesting that P49 is useful as a carrier to target foreign proteins to EMVs for production of artificial EMVs with desired functions. This method is potentially useful for the preparation of designed vaccines and is described in detail in this chapter.