A new small RNA virus persistently infecting an established cell line of Galleria mellonella, induced by a heterologous infection
A new small RNA virus persistently infecting an established cell line of Galleria mellonella, induced by a heterologous infection
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DOI：
10.1006/jipa.1996.4617
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发表时间：
1997-01-01
影响因子：
3.4
通讯作者：
Giannotti, J
中科院分区：
文献类型：
--
作者：
Lery, X;Fediere, G;Giannotti, J
用分离自玉米茎螟小RNA样病毒（MSBV）感染大蜡螟细胞系，发现该病毒在大蜡螟细胞系中持续感染。新病毒与MSBV完全不同，命名为G。Mellonella细胞系病毒（GmclV）诱导显著的细胞病变效应，并且也被认为在体内有效。GmclV是一种直径为29 nm的等轴病毒，具有分子量为2.9 x 10（6）Da的单链RNA和poly（A）区。其衣壳仅由34，500和32，500 Da的两种主要多肽组成，并且未检测到次要条带。GmclV的特征不允许我们有把握地对其进行分类。尽管它尚未被鉴定为小核糖核酸病毒，但它可以被分类为小核糖核酸病毒科的小RNA病毒组。G.由于在体内和体外分离了属于同一家族的两种不同的持久性病毒，大蜡螟属代表了一个非常有趣的模型，以进一步理解持久性和诱导的一般现象。(C)北京：科学出版社.
A persistent infection in a Galleria mellonella cell line was revealed when infected with a maize stem borer picorna-like virus isolated on Sesamia cretica (MSBV). The new virus, completely different from the MSBV, is designated as G. mellonella cell line virus (GmclV), induces spectacular cytopathic effects, and is also considered efficient in vivo. The GmclV is a 29-nm-diameter isometric virus, with single-strand RNA of 2.9 x 10(6) Da molecular weight with a poly(A) tract. Its capsid is constituted of only two major polypeptides, of 34,500 and 32,500 Da, and no minor bands could be detected. The characteristics of the GmclV do not permit us to classify it with assurance. Even though it has not yet been identified as a picornavirus, it can be classified in the small RNA virus group of the Picornaviridae. G. mellonella represents a very interesting model, owing to the fact that two different persistent viruses belonging to the same family were isolated in vivo and in vitro, to further the understanding of the general phenomenon of persistency and induction. (C) 1997 Academic Press.