Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I.
Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I.
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DOI：
10.1093/nar/gkl101
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发表时间：
2006
影响因子：
14.9
通讯作者：
Loidl P
中科院分区：
文献类型：
--
作者：
Meraner J;Lechner M;Loidl A;Goralik-Schramel M;Voit R;Grummt I;Loidl P
上游结合因子UBF是RNA聚合酶I转录的激活剂，通过磷酸化和乙酰化进行翻译后修饰。我们发现，在NIH 3 T3细胞中，UBF在S期被乙酰化，而在G1期没有乙酰化。为了评估乙酰化在调节UBF活性中的作用，我们建立了诱导性过表达HDAC 1的NIH 3 T3细胞系。在体内和体外，HDAC 1有效地使UBF低乙酰化。免疫沉淀与抗体对Pol I相关因子PAF 53共沉淀UBF在模拟细胞，但不是在细胞过表达HDAC 1。下拉实验表明，乙酰化的UBF增强与Pol I的相互作用。与UBF的乙酰化对于PAF 53的缔合和Pol I的募集是重要的一致，与rDNA和pre-rRNA合成相关的Pol I的水平在过表达HDAC 1的细胞中降低。结果表明，乙酰化和去乙酰化的UBF调节rRNA的合成在细胞周期的进程。
The upstream binding factor UBF, an activator of RNA polymerase I transcription, is posttranslationally modified by phosphorylation and acetylation. We found that in NIH3T3 cells, UBF is acetylated in S-phase but not in G1-phase. To assess the role of acetylation in regulation of UBF activity, we have established an NIH3T3 cell line that inducibly overexpresses HDAC1. Both in vivo and in vitro, HDAC1 efficiently hypoacetylates UBF. Immunoprecipitation with antibodies against the Pol I-associated factor PAF53 co-precipitated UBF in mock cells but not in cells overexpressing HDAC1. Pull-down experiments showed that acetylation of UBF augments the interaction with Pol I. Consistent with acetylation of UBF being important for association of PAF53 and recruitment of Pol I, the level of Pol I associated with rDNA and pre-rRNA synthesis were reduced in cells overexpressing HDAC1. The results suggest that acetylation and deacetylation of UBF regulate rRNA synthesis during cell cycle progression.
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影响因子：
8
作者：
Hannan, KM;Hannan, RD;Rothblum, LI
通讯作者：
Rothblum, LI
DOI：
10.1016/0167-4838(96)00069-6
发表时间：
1996-09-05
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
Lechner, T;Lusser, A;Loidl, P
通讯作者：
Loidl, P
影响因子：
64.8
作者：
Choi, CH;Hiromura, M;Usheva, A
通讯作者：
Usheva, A
影响因子：
14.9
作者：
Hirschler-Laszkiewicz, I;Cavanaugh, A;Rothblum, LI
通讯作者：
Rothblum, LI
影响因子：
5.3
作者：
Ciarmatori, S;Scott, PH;White, RJ
通讯作者：
White, RJ