Multiple target-site mutations occurring in lepidopterans confer resistance to diamide insecticides
Multiple target-site mutations occurring in lepidopterans confer resistance to diamide insecticides
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鳞翅目动物中发生的多个靶位点突变赋予对二酰胺杀虫剂的抗性
DOI：
10.1016/j.ibmb.2020.103367
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发表时间：
2020-06-01
影响因子：
3.8
通讯作者：
Wu, Shun-Fan
中科院分区：
文献类型：
--
作者：
Huang, Jing-Mei;Rao, Cong;Wu, Shun-Fan
联胺抗性表型已经在田间进化，这种抗性被归因于一些鳞翅目害虫的靶点突变。在本研究中，我们记录了2016-2018年在中国7省的二化螟对氯氰菊酯的抗性状况。为了研究已知的鳞翅目昆虫靶点突变的可能作用，我们对具有不同水平的联胺抗性的抑制隐翅虫的RyR基因的各个区域进行了测序。结果表明，小菜蛾中存在I4758M(对应于小菜蛾14790M)、Y4667D/C(根据C.suppressalis编号)、G4915E(对应于小菜蛾G4946E)和一个新的Y4891F(根据C.suppressalis编号)RyR靶点突变。通过对8个基因组修饰的黑腹果蝇品系进行联胺毒性生物测定，进一步研究了这些突变的贡献。研究表明，携带Y4667D突变(对应于抑制性隐翅虫Y4667D和I4758M同时突变)的基因组修饰果蝇对氯氰菊酯(1542.8倍)、氰甲苯丙醇(487.9倍)和四氯苯丙醇(290.1倍)具有较高的抗药性。M4758I和G4915E同时突变(对应于抑制性隐翅虫的单一G4915E突变)对氯苯丙胺(153.1倍)和氰乙丙胺(323.5倍)具有较高的耐药率，而对氟苯二胺(28.9倍)和四氯苯丙胺(25.2倍)的耐药率相对较低。这些发现表明，RyR的多个点突变导致了抑制葡萄球菌对联胺的耐药性。这些结果有助于更好地了解昆虫对联胺的抗性机制，并为深入了解RyR靶点突变对昆虫的影响提供依据。
Diamide resistant phenotypes have evolved in the field and the resistance has been attributed to target-site mutations in some lepidopteran pests. In this study, we documented the resistance status of Chilo suppressalis to chlorantraniliprole during 2016-2018 in seven provinces of China. To investigate the possible role of target-site mutations as known from lepidopterans, we sequenced respective domains of the RyR gene of C. suppressalis with different levels of diamide resistance. The results revealed that I4758M (corresponding to 14790M in P. xylostella), Y4667D/C (numbered according to C. suppressalis), G4915E (corresponding to G4946E in P. xylostella), and one novel Y4891F (numbered according to C. suppressalis) RyR target-site mutations were present. The contribution of these mutations was further investigated by diamide toxicity bioassays with eight genome modified Drosophila melanogaster lines. The study showed that genome modified flies bearing the Y4667D mutation (corresponding to the Y4667D and I4758M simultaneous mutation in C. suppressalis) exhibited high resistance ratios to chlorantraniliprole (1542.8-fold), cyantraniliprole (487.9-fold) and tetrachlorantraniliprole (290.1-fold). The M4758I and G4915E simultaneous mutations (corresponding to single G4915E mutation in C. suppressalis) showed high resistance ratios to chlorantraniliprole (153.1-fold) and cyantraniliprole (323.5-fold), and relatively low resistance to flubendiamide (28.9-fold) and tetrachlorantraniliprole (25.2-fold). These findings suggest that multiple point mutations in RyR confer diamide resistance of C. suppressalis. The results contribute to a better understanding of insect diamide resistance mechanisms and provide insights on the impact of RyR target-site mutations in insects.