AUTOPHAGY OF MITOCHONDRIA IN RAT BONE-MARROW ERYTHROID-CELLS - RELATION TO NUCLEAR EXTRUSION
AUTOPHAGY OF MITOCHONDRIA IN RAT BONE-MARROW ERYTHROID-CELLS - RELATION TO NUCLEAR EXTRUSION
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DOI：
10.1007/bf00214924
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
VERWILGHEN, RL
中科院分区：
文献类型：
--
作者：
HEYNEN, MJ;TRICOT, G;VERWILGHEN, RL
用电镜体视学方法对雄性Wistar大鼠骨髓中的晚期成红细胞和网织红细胞进行了研究。分别分析具有核挤出形态学体征的晚期成红细胞（EN+成红细胞）和不具有这些体征的晚期成红细胞（EN-成红细胞）。线粒体，自噬体，自噬线粒体，结果表明：（1）在（EN-）-成红细胞向（EN+）-成红细胞成熟的过程中，细胞质中线粒体的体积密度下降了34（p< 0.001），并且在从（EN+）-成红细胞分化为网织红细胞期间增加60%（p<0.001），（2）在从（EN-）-成红细胞到（EN+）-成红细胞的成熟过程中，细胞质中自噬体的体积密度增加了5倍（p<0.01），而该参数的值在随后分化为网织红细胞期间基本保持不变，（3）在（EN-）-成红细胞的自噬体内未发现线粒体，而线粒体分别占26%和35%，自噬体体积的（EN+）-我们的研究结果表明，线粒体的自噬作用开始于核挤出的时刻，并在骨髓网织红细胞中继续。
Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN+erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitochondria, autophagosomes, autophagocytosed mitochondria, autophagocytosed cytoplasm and degraded material inside autophagosomes were calculated per unit volume of cytoplasm.The results demonstrate that (1) the volume density of mitochondria in the cytoplasm decreases by 34% during maturation from (EN-)- to (EN+)-erythroblasts (p< 0.001) and by 60% during differentiation from (EN+)-erythroblasts to reticulocytes (p<0.001), (2) a fivefold increase in the volume density of autophagosomes in the cytoplasm is noted during maturation from (EN-)- to (EN+)-erythroblasts (p<0.01), whereas the value of this parameter remains essentially unchanged during the subsequent differentiation to reticulocytes, (3) no mitochondria are found inside autophagosomes of (EN-)-erythroblasts, whereas mitochondria occupy 26% and 35%, respectively, of the autophagosomal volume in (EN+)-erythroblasts and in reticulocytes.Our results show that autophagocytosis of mitochondria starts at the moment of nuclear extrusion and continues in the bone marrow reticulocytes.