METABOLISM OF THE PHOSPHOINOSITIDES IN GUINEA‐PIG BRAIN SYNAPTOSOMES
METABOLISM OF THE PHOSPHOINOSITIDES IN GUINEA‐PIG BRAIN SYNAPTOSOMES
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豚鼠脑突触体中磷酸肌醇的代谢
DOI：
10.1111/j.1471-4159.1969.tb05989.x
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发表时间：
1969
影响因子：
4.7
通讯作者：
J. Hawthorne
中科院分区：
文献类型：
--
作者：
J. Harwood;J. Hawthorne
摘要-研究了一些与肌醇脂代谢有关的酶在受损豚鼠脑突触体亚组分中的亚细胞分布。这些酶分别是CDP-二甘油酯：肌醇磷脂酸酯转移酶、磷脂酰肌醇转移酶、磷脂酰肌醇转移酶、二磷脂酰肌醇转移酶、磷脂酰肌醇转移酶、磷脂酰肌醇肌醇转移酶、肌醇肌比较了水处理的突触体亚组份中磷脂酰肌醇激酶的分布与其他质膜酶的分布。用Triton X-100部分溶解突触体后，检测磷脂酰肌醇激酶和其他几种酶的活性。
Abstract— The subcellular distribution of a number of enzymes concerned with inositol lipid metabolism has been studied in sub‐fractions of disrupted guinea‐pig brain synaptosomes. The enzymes were CDP‐diglyceride: inositol phosphatidate transferase, phospha‐tidylinositol kinase, diphosphoinositide kinase, diphosphoinositide phosphomonoesterase and diphosphoinositide diesterase. The distribution of phosphatidylinositol kinase in sub‐fractions from water‐treated synaptosomes was compared with that of other plasma membrane enzymes. After partial solubilization of synaptosomes by Triton X‐100 the activities of phosphatidylinositol kinase and several other enzymes were examined.