Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin
Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin
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单核细胞增生李斯特氏菌溶血素的心脏毒性和致死作用
DOI：
10.1128/iai.1.4.373-379.1970
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发表时间：
1970
影响因子：
3.1
通讯作者：
C. Sword
中科院分区：
文献类型：
--
作者：
G. Kingdon;C. Sword
本文研究了单核细胞增多性李斯特菌溶血素对CD-1小鼠的心脏毒性和致死作用。静脉注射100个完全溶血单位(CHU)，在4~5分钟内致死率为100%。从40到50个CHU的剂量会导致大约50%的动物死亡。肾上腺素能阻滞剂和抗组胺药不能保护小鼠免于死亡，因此表明死亡不是由于溶血素释放血管活性物质所致。静脉注射溶血素后，血浆肌酸磷酸激酶水平升高，提示有肌病，可能是心肌病变。溶血素处理的小鼠的心电图显示心率和节律发生严重变化，表明收缩和起搏器心脏组织受到损害。此外，有迹象表明钾水平增加会影响心脏。据推测，死亡是由于心脏肌肉功能受损和电骤停。通过预先将溶血素与胆固醇孵育、加热或不用半胱氨酸重新激活制剂，可以防止心脏毒性和致命性影响。
Cardiotoxic and lethal effects of Listeria monocytogenes hemolysin were studied in CD-1 mice injected with varying doses of hemolysin. Intravenous injection of 100 complete hemolytic units (CHU) caused 100% lethality within 4 to 5 min. Doses ranging from 40 to 50 CHU caused death of approximately 50% of the animals. Adrenergic blocking agents and antihistamine failed to protect mice against lethality and thereby suggested that death was not due to release of vasoactive agents by hemolysin. Plasma levels of creatine phosphokinase increased after intravenous administration of hemolysin and suggested myopathy, possibly of the myocardium. Electrocardiograms from hemolysin-treated mice indicated serious alterations in heart rate and rhythm, suggesting damage to contractile and pacemaker cardiac tissue. In addition, there were indications of increased potassium levels influencing the heart. Presumably, death was due to functional damage to heart muscle and electrical arrest. The cardiotoxic and lethal effects could be prevented by prior incubation of hemolysin with cholesterol, heating, or failure to reactivate the preparation with cysteine.