NF-Ya protein delivery as a tool for hematopoietic progenitor cell expansion.
NF-Ya protein delivery as a tool for hematopoietic progenitor cell expansion.
复制标题
NF-Ya 蛋白递送作为造血祖细胞扩增的工具。
DOI：
10.1007/978-1-61779-980-8_23
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Emerson,StephenG
中科院分区：
文献类型：
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作者：
Domashenko,AlevtinaD;Wiener,Susan;Emerson,StephenG
原代造血细胞的临床治疗潜力，无论是未经修饰的还是通过基因修饰改变的，刺激了对技术的探索，使大量造血前体，更原始的祖细胞，也许造血干细胞（HSC）本身的生产。体外培养条件的改变促进祖细胞扩增包括多肽细胞因子、小分子和转录因子的组合。在这里，我们描述了使用与基于tat的蛋白质转录域相关的转录因子的方法，结合细胞因子和无血清培养条件来刺激原代细胞的增殖。经TAT-NF-Ya融合蛋白处理并在体外培养1个月的人外周血（PB） CD34+细胞的增殖能力是仅含细胞因子的培养细胞的4倍，包括所有成熟的造血细胞的产量增加。这些结果和技术应该适用于利用蛋白质转导体外生成造血细胞的多种应用。
The clinical potential of therapeutic quantities of primary hematopoietic cells, either unmodified or altered via genetic modification, has stimulated the search for techniques that allow the production of large numbers of hematopoietic precursors, more primitive progenitors, and perhaps hematopoietic stem cells (HSC) themselves. Modifications of in vitro culture conditions to promote progenitor cell expansion have included combinations of polypeptide cytokines, small molecules, and transcription factors. Here we describe the methods for use of the transcription factor linked to a TAT-based protein transcription domain, in combination with cytokines and serum-free culture condition to stimulate the proliferation of primary cells. Human peripheral blood (PB) CD34+cells treated with TAT-NF-Ya fusion protein and grown in vitro for 1 month proliferate four times more than did cells in cultures that contained only cytokines, including increased production of hematopoietic cells of all maturities. These results and techniques should be suitable for multiple applications of ex vivo generation of hematopoietic cells using protein transduction.