Hepatocellular carcinomas in cirrhotic and noncirrhotic human livers share angiogenic characteristics.
Hepatocellular carcinomas in cirrhotic and noncirrhotic human livers share angiogenic characteristics.
复制标题
DOI：
10.1245/s10434-009-0900-z
复制
发表时间：
2010-06
影响因子：
3.7
通讯作者：
de Jong KP
中科院分区：
文献类型：
--
作者：
Zeng W;Gouw AS;van den Heuvel MC;Molema G;Poppema S;van der Jagt EJ;de Jong KP
抗血管生成药物索拉非尼已被证明是治疗肝硬化患者肝细胞癌（HCC）的有效方法。如果两种肿瘤类型的血管生成特性具有可比性，它也可能对非肝硬化 HCC 有效。本研究的目的是比较内皮细胞动力学、微血管密度 (MVD) 和血管成熟度作为肝硬化和非肝硬化肝脏中人类 HCC 血管生成的间接标志物。在三级护理环境中，对 70 个连续的 HCC 肿瘤进行了内皮细胞动力学分析。 CD34 用于识别肿瘤微血管，双免疫标记 Ki67/CD34 和激活的 caspase-3/CD34 用于评估内皮细胞增殖和凋亡，α-平滑肌肌动蛋白/CD34 用于评估周细胞覆盖。在肝硬化 (n = 33) 和非肝硬化 HCC (n = 37) 中对这些特征进行了比较。微血管密度与造影增强的动脉期和门脉期动态四相 CT 扫描获得的血管丰富的放射学体征相关。肝硬化和非肝硬化HCC的微血管主要是成熟的。两组的内皮细胞更新率均较低且 MVD 没有差异。 MVD 与静脉侵犯、肿瘤大小以及肿瘤细胞或内皮细胞的更新之间没有相关性。 MVD 与 CT 扫描门静脉期造影剂冲洗呈负相关。在移植患者中，MVD 与生存率无关，而在肝切除术后的患者中，高 MVD 与更好的预后相关。肝硬化和非肝硬化肝脏中HCC的血管生成特征具有显着的相似性。
The antiangiogenic drug sorafenib has been shown to be an effective treatment for hepatocellular carcinoma (HCC) in patients with liver cirrhosis. It might also be effective in noncirrhotic HCC provided that the angiogenic properties of both tumor types are comparable. The aim of this study is to compare endothelial cell dynamics, microvessel density (MVD), and vessel maturation as indirect markers of angiogenesis in human HCC in cirrhotic and noncirrhotic livers. In a tertiary care setting, 70 consecutive HCC tumors were analyzed for endothelial cell dynamics. CD34 was applied to identify tumor microvessels, double immunolabeling Ki67/CD34 and activated caspase-3/CD34 to assess endothelial cell proliferation and apoptosis, and α-smooth muscle actin/CD34 for pericyte coverage. These characteristics were compared in cirrhotic (n = 33) and noncirrhotic HCCs (n = 37). Microvessel density was correlated with radiological signs of hypervascularity as obtained with dynamic four-phase CT scans during the arterial and portal phase of contrast enhancement. Microvessels in cirrhotic and noncirrhotic HCC were mainly mature. In both groups endothelial cell turnover was low and MVD was not different. There was no correlation between MVD and venous invasion, tumor size, and turnover of tumor cells or endothelial cells. MVD was negatively correlated with contrast washout in the portal venous phase of CT scanning. In transplanted patients, MVD was not correlated with survival, whereas in patients after liver resection a high MVD was associated with a better prognosis. Angiogenic characteristics of HCC in cirrhotic and noncirrhotic livers have a remarkable similarity.
登录
查看更多内容
影响因子：
45.3
作者：
Poon, RTP;Ng, IOL;Wong, J
通讯作者：
Wong, J
影响因子：
158.5
作者：
Llovet, Josep M.;Ricci, Sergio;Bruix, Jordi
通讯作者：
Bruix, Jordi
影响因子：
2.6
作者：
Lubrano, Jean;Huet, Emmanuel;Scotte, Michel
通讯作者：
Scotte, Michel
影响因子：
13.5
作者：
Mitsuhashi, N;Shimizu, H;Miyazaki, M
通讯作者：
Miyazaki, M
影响因子：
11.5
作者：
Leenders, WPJ;Küsters, B;de Waal, R
通讯作者：
de Waal, R