Gene Expression Analysis of Environmental Temperature and High-Fat Diet-Induced Changes in Mouse Supraclavicular Brown Adipose Tissue.
Gene Expression Analysis of Environmental Temperature and High-Fat Diet-Induced Changes in Mouse Supraclavicular Brown Adipose Tissue.
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环境温度和高脂饮食诱导的小鼠上乳棕色脂肪组织变化的基因表达分析。
DOI：
10.3390/cells10061370
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发表时间：
2021-06-02
期刊：
影响因子：
6
通讯作者：
Chen MH
中科院分区：
文献类型：
--
作者：
Shi Y;Zhai H;John S;Shen YT;Ran Y;Hoang G;Chen MH
肥胖是脂肪组织的失调，是与许多疾病相关的主要健康危险因素。棕色脂肪组织（BAT）介导的生热作用可以潜在地调节能量消耗，使其成为对抗肥胖的有吸引力的治疗靶点。在这里，我们与其他脂肪库（包括肩胛间 BAT (iBAT)）相比，描述了冷暴露、热中性和高脂饮食 (HFD) 对小鼠锁骨上 BAT (scBAT) 形态和 BAT 相关基因表达的影响。 scBAT 与 iBAT 一样对冷诱导的生热作用敏感，并且在热中性条件下表现出降低的生热效应。虽然 scBAT 和 iBAT 都对寒冷敏感，但参与营养加工的基因表达却不同。 scBAT 还显示出较少的储存体重增加和更多的单脂脂肪细胞，而 BAT 生热基因（例如 Ucp1）的表达在我们的 HFD 喂养方案下，在环境温度和热中性温度下与 iBAT 保持相似或增加更多。总之，这些发现表明，除了与人类 scBAT 的解剖学相似性之外，小鼠 scBAT 还具有与其他脂肪库不同的产热特征。最后，这项研究还描述了一种以前未知的小鼠深颈 BAT (dnBAT) 库，该库在冷暴露和热中性条件下表现出与 scBAT 相似的生热特性。
Obesity, a dysregulation of adipose tissue, is a major health risk factor associated with many diseases. Brown adipose tissue (BAT)-mediated thermogenesis can potentially regulate energy expenditure, making it an attractive therapeutic target to combat obesity. Here, we characterize the effects of cold exposure, thermoneutrality, and high-fat diet (HFD) feeding on mouse supraclavicular BAT (scBAT) morphology and BAT-associated gene expression compared to other adipose depots, including the interscapular BAT (iBAT). scBAT was as sensitive to cold induced thermogenesis as iBAT and showed reduced thermogenic effect under thermoneutrality. While both scBAT and iBAT are sensitive to cold, the expression of genes involved in nutrient processing is different. The scBAT also showed less depot weight gain and more single-lipid adipocytes, while the expression of BAT thermogenic genes, such as Ucp1, remained similar or increased more under our HFD feeding regime at ambient and thermoneutral temperatures than iBAT. Together, these findings show that, in addition to its anatomical resemblance to human scBAT, mouse scBAT possesses thermogenic features distinct from those of other adipose depots. Lastly, this study also characterizes a previously unknown mouse deep neck BAT (dnBAT) depot that exhibits similar thermogenic characteristics as scBAT under cold exposure and thermoneutrality.
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影响因子：
2.5
作者：
Cui X;Nguyen NL;Zarebidaki E;Cao Q;Li F;Zha L;Bartness T;Shi H;Xue B
通讯作者：
Xue B
影响因子：
6.5
作者：
Duarte, Joao A. G.;Carvalho, Filipa;Burgess, Shawn C.
通讯作者：
Burgess, Shawn C.
DOI：
10.1152/ajpendo.00023.2015
发表时间：
2015-06-15
影响因子：
5.1
作者：
de Jong, Jasper M. A.;Larsson, Ola;Nedergaard, Jan
通讯作者：
Nedergaard, Jan
影响因子：
29
作者：
Feldmann, Helena M.;Golozoubova, Valeria;Nedergaard, Jan
通讯作者：
Nedergaard, Jan
影响因子：
16.6
作者：
Ferrari A;Longo R;Fiorino E;Silva R;Mitro N;Cermenati G;Gilardi F;Desvergne B;Andolfo A;Magagnotti C;Caruso D;Fabiani E;Hiebert SW;Crestani M
通讯作者：
Crestani M