Estrogen regulates GFAP-expression in specific subnuclei of the female rat interpeduncular nucleus: a potential role for estrogen receptor beta.
Estrogen regulates GFAP-expression in specific subnuclei of the female rat interpeduncular nucleus: a potential role for estrogen receptor beta.
复制标题
雌激素调节雌性大鼠脚间核特定亚核中的 GFAP 表达：雌激素受体 β 的潜在作用。
DOI：
10.1016/s0006-8993(02)03771-x
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发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
Belcher,ScottM
中科院分区：
文献类型：
--
作者：
Zsarnovszky,Attila;Smith,Todd;Hajos,Ferenc;Belcher,ScottM
我们以前证明，在大鼠，星形胶质细胞胶质细胞酸性蛋白（GFAP）的脚间核（IPN）的表达是响应睾酮和在女性的GFAP免疫反应性（IR）的强度遵循周期性的激素变化的动情周期。本研究的目的是测试在没有其他卵巢激素的情况下，17β-雌二醇（E2）是否可以影响IPN的单个亚核内GFAP的表达，并确定IPN中雌激素受体β（ERβ）的细胞分布。用定量表面密度分析比较E2或溶剂处理后24 h卵巢切除雌性大鼠IPN不同前后（AP）水平GFAP-IR的强度。雌激素治疗导致AP时IPN的头外侧亚核GFAP-IR显著增加：-5.60，外侧亚核、背外侧亚核、背内侧亚核和中央亚核为-6.04，外侧亚核为-6.72。在-5.80和-6.30处未观察到显著差异。这些结果表明，E2，在其他卵巢激素的情况下，调节选择IPN亚核内的GFAP表达，这些影响是依赖于位置沿着AP轴。为了确定ERβ是否是所观察到的雌激素效应的可能介质，对IPN的相邻切片对进行ERβ或GFAP免疫染色。采用“镜像”法，在GFAP免疫阳性的星形胶质细胞胞浆和GFAP免疫阴性的神经元胞核中检测到ERβ-IR。提示E2可能通过ERβ介导的机制直接调节IPN中GFAP的表达。
We previously demonstrated that in rat, astrocytic glial fibrillary acidic protein- (GFAP) expression in the interpeduncular nucleus (IPN) was responsive to testosterone and in females the intensity of GFAP-immunoreactivity (IR) followed the periodic hormonal changes of the estrous cycle. The aim of this study was to test whether 17β-estradiol (E2), in the absence of other ovarian hormones, can influence GFAP-expression within individual subnuclei of the IPN and to determine the cellular distribution of estrogen receptor β (ERβ) in the IPN. Quantitative surface-density analysis was used to compare the intensity of GFAP-IR at different anterio-posterior (AP) levels of the IPN in ovariectomized female rats 24 h after treatment with E2or vehicle. Estrogen-treatment resulted in a significant increase in GFAP-IR in the rostrolateral subnucleus of the IPN at AP: −5.60, in the lateral-, dorsolateral-, dorsomedial- and central subnuclei at −6.04 and in the lateral subnucleus at −6.72. No significant differences were observed at −5.80 and −6.30. These results indicate that E2, in the absence of other ovarian hormones, modulates GFAP-expression within select IPN subnuclei and that these affects are dependent on position along the AP axis. To determine whether ERβ was a possible mediator of the observed estrogenic effects, adjacent section pairs of the IPN were immunostained for ERβ or GFAP. Using the ‘mirror’ method, ERβ-IR was detected in the cytoplasm of GFAP-immunopositive astroglia and in the nuclei of GFAP-immunonegative neurons. These findings suggest that in the IPN, E2may directly modulate GFAP-expression through ERβ-mediated mechanisms.