Programmable Sensors of 5-Hydroxymethylcytosine
Programmable Sensors of 5-Hydroxymethylcytosine
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DOI：
10.1021/ja506022t
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发表时间：
2015-01-14
影响因子：
15
通讯作者：
Summerer, Daniel
中科院分区：
文献类型：
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作者：
Kubik, Grzegorz;Batke, Sabrina;Summerer, Daniel
5-羟甲基胞嘧啶（hmC）是哺乳动物基因组的第六个碱基，是一种具有重要生物学功能的表观遗传标记。我们报告工程，可编程转录激活因子样效应（TALE）作为第一个DNA结合受体分子，提供直接的，个人的选择性胞嘧啶（C），5-甲基胞嘧啶（mC），和hmC在用户定义的DNA序列。鉴于TALE在体外和体内可编程靶向DNA序列的广泛适用性，这为表观遗传学研究提供了广阔的前景。
5-Hydroxymethylcytosine (hmC), the sixth base of the mammalian genome, is increasingly recognized as an epigenetic mark with important biological functions. We report engineered, programmable transcription-activator-like effectors (TALEs) as the first DNA-binding receptor molecules that provide direct, individual selectivities for cytosine (C), 5-methylcytosine (mC), and hmC at user-defined DNA sequences. Given the wide applicability of TALEs for programmable targeting of DNA sequences in vitro and in vivo, this provides broad perspectives for epigenetic research.