Use of GelMA for 3D printing of cardiac myocytes and fibroblasts.
Use of GelMA for 3D printing of cardiac myocytes and fibroblasts.
复制标题
DOI：
10.2217/3dp-2018-0017
复制
发表时间：
2019-03-01
期刊：
影响因子：
--
通讯作者：
Sarvazyan, Narine A
中科院分区：
文献类型：
--
作者：
Koti, Priyanka;Muselimyan, Narine;Sarvazyan, Narine A
目的：要3D打印心脏组织，必须了解打印过程如何影响两种主要类型的心脏细胞。材料与方法：采用乳酸脱氢酶法、LIVE/DEAD法、生物发光成像法和形态学法检测明胶甲基丙烯酰（GelMA）浓度、挤出机压力和紫外线照射时间对心肌细胞和成纤维细胞存活的影响。结果与结论：与3D成纤维细胞负载的GelMA构建体相比，3D打印心肌细胞负载的GelMA构建体中的细胞存活对挤出机压力和GelMA浓度更为敏感。两种结构中的细胞都受到紫外线固化步骤的不利影响。使用混合细胞群和用纤维连接蛋白富集生物链接制剂可改善心肌细胞的存活和扩散。
AIM: To 3D print heart tissue, one must understand how the main two types of cardiac cells are affected by the printing process.MATERIALS & METHODS: Effects of gelatin methacryloyl (GelMA) concentration, extruder pressure and duration of UV exposure on survival of cardiac myocytes and fibroblasts were examined using lactate dehydrogenase and LIVE/DEAD assays, bioluminescence imaging and morphological assessment.RESULTS & CONCLUSION: Cell survival within 3D printed cardiomyocyte-laden GelMA constructs was more sensitive to extruder pressure and GelMA concentrations than within 3D fibroblast-laden GelMA constructs. Cells within both types of constructs were adversely impacted by the UV curing step. Use of mixed cell populations and enrichment of bioink formulation with fibronectin led to an improvement of cardiomyocyte survival and spreading.