Transplantation of autologous oral mucosal epithelial cell sheets inhibits the development of acquired external auditory canal atresia in a rabbit model
Transplantation of autologous oral mucosal epithelial cell sheets inhibits the development of acquired external auditory canal atresia in a rabbit model
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自体口腔粘膜上皮细胞片移植抑制兔模型获得性外耳道闭锁的发展
DOI：
10.1016/j.actbio.2020.04.031
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yuichiro Yaguchi
中科院分区：
文献类型：
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作者：
Taro Inagaki ;Tsunetaro Morino ;Ryo Takagi ;Masayuki Yamato ;Izumi Koizuka ;Yuichiro Yaguchi
后天性外耳道闭锁的特征是耳道内纤维组织形成、听力损失和慢性耳病。虽然这种疾病可以手术治疗，但复发率很高。本研究探讨自体口腔黏膜上皮细胞片是否可作为治疗耳道闭锁的一种新方法。我们通过耳道皮肤的解剖，成功地建立了获得性外耳道闭锁的兔模型。在该模型中的内窥镜和组织学结果表明，闭锁在4周内发展，并且在皮肤解剖后立即放置聚乙醇酸片不会抑制闭锁。相比之下，移植自体口腔粘膜上皮细胞片，这已制造的温度响应插入无饲养层的培养，防止闭锁的发展，在4周内皮肤解剖后。后天性外耳道闭锁手术治疗后自体上皮细胞片移植可能是降低疾病复发风险的一种有前途的新方法。重要性声明后天性外耳道闭锁的特征是耳道内纤维组织形成，导致听力损失和慢性耳病。虽然手术治疗是可行的，但复发率很高。本研究通过耳道皮肤的解剖，成功地建立了获得性外耳道闭锁的兔模型。此外，我们利用这种新的动物模型，以探讨是否自体口腔粘膜上皮细胞片移植可作为一种新的治疗耳道闭锁。我们的研究结果提高了耳道闭锁手术治疗后自体上皮细胞片移植可能是一种有前途的新方法，以减少疾病复发的风险。
Acquired external auditory canal atresia is characterized by fibrous tissue formation in the ear canal, hearing loss and chronic otorrhea. Although the disease can be treated surgically, the recurrence rate is high. This study explored whether autologous oral mucosal epithelial cell sheets could be used as a novel therapy for ear canal atresia. We succeeded in generating a rabbit model of acquired external auditory canal atresia by dissecting the skin of the ear canal. Endoscopic and histological findings in this model indicated that atresia developed over a 4-week period and was not inhibited by the placement of polyglycolic acid sheets immediately after skin dissection. By contrast, transplantation of autologous oral mucosal epithelial cell sheets, which had been fabricated by culture on temperature-responsive inserts without a feeder layer, prevented the development of atresia during the 4-week period after skin dissection. Transplantation of autologous epithelial cell sheets after surgical treatment of acquired external auditory canal atresia could be a promising new method to reduce the risk of disease recurrence.Statement of SignificanceAcquired external auditory canal atresia is characterized by fibrous tissue formation in the ear canal, which leads to hearing loss and chronic otorrhea. Although surgical treatments are available, the recurrence rate is high. In this study, we successfully generated a rabbit model of acquired external auditory canal atresia by dissecting the skin of the ear canal. Furthermore, we utilized this new animal model to investigate whether the transplantation of autologous oral mucosal epithelial cell sheets could be used as a novel therapy for ear canal atresia. Our results raise the possibility that the transplantation of autologous epithelial cell sheets after surgical treatment of ear canal atresia could be a promising new method to reduce the risk of disease recurrence.