The stimulation of microsomal azoreduction by flavins.
The stimulation of microsomal azoreduction by flavins.
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黄素刺激微粒体偶氮还原。
DOI：
10.1016/0304-4165(82)90087-3
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Peisach,J
中科院分区：
文献类型：
--
作者：
Fujita,S;Peisach,J
一氧化碳可抑制大鼠肝微粒体还原偶氮染料阿马兰斯红，抑制率约为90%，表明该反应主要依赖于细胞色素P-450。该反应的还原当量由NADPH提供。该反应被核黄素、FMN和FAD以及甲基紫精刺激。大部分的刺激反应不被CO阻断，表明存在依赖于细胞色素P-450的电子转移途径。大鼠肝微粒体可还原FAD，还原当量由NADPH提供。这样产生的FADH 2被阿马兰斯红迅速氧化，因此每还原一个阿马兰斯红，就有两个FADH 2被氧化。在不存在微粒体的情况下形成的光化学制备的FADH 2观察到相同的化学计量。
The reduction of the azo dye, amaranth, by rat liver microsomes is inhibited about 90% by carbon monoxide, suggesting that the reaction largely depends on cytochromeP-450. Reducing equivalents for this reaction are supplied by NADPH. This reaction is stimulated by riboflavin, FMN and FAD, as well as by methylviologen. A large fraction of the stimulated reaction is not blocked by CO, indicating that there is a pathway of electron transfer which is dependent of cytochromeP-450. Rat liver microsomes can reduce FAD, with reducing equivalents supplied by NADPH. The FADH2thus produced is quickly oxidized by amaranth so that two FADH2are oxidized for every amaranth reduced. The same stoichiometry is observed with photochemically prepared FADH2, formed in the absence of microsomes.