The normal human chondro-osseous junctional region: evidence for contact of uncalcified cartilage with subchondral bone and marrow spaces
The normal human chondro-osseous junctional region: evidence for contact of uncalcified cartilage with subchondral bone and marrow spaces
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DOI：
10.1186/1471-2474-7-52
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发表时间：
2006-06-20
影响因子：
2.3
通讯作者：
McClure, John
中科院分区：
文献类型：
--
作者：
Lyons, Tim J.;McClure, Sheena F.;McClure, John
背景资料：关节的软骨-骨结合区特别复杂，可以认为是由最深的非钙化软骨层、潮标、钙化软骨层、（钙化软骨和软骨下骨之间的）薄骨水泥线和软骨下骨组成。一个详细的知识的结构，功能和病理生理学的正常chondrooseous交界处是必不可少的了解的发病机制的骨关节病。方法：全层标本从人类膝关节进行处理，石蜡包埋。从正常关节内侧胫骨平台的块的软骨-骨结合区取100个连续切片（10 μ m厚）。它们用苏木精和伊红染色并拍照。对于每个第10个连续组织切片的简单物理重建图像，打印包含软骨-骨结合区的显微照片区域，然后将其重叠，以创建该区域的三维（3D）模型。一个3D重建也使用计算机modeling.Results：组织化学染色显示一些情况下，未钙化的软骨，划定的潮标，浸到钙化的软骨，并在地方，dumping到软骨下骨和骨髓空间。在二维（2D）切片中，可见含有软骨细胞的小面积未钙化软骨（虚拟岛），明显埋藏在钙化基质中。简单的物理3D重建证实了这些未钙化软骨的膨胀与IV区的软骨是连续的，并证明了未钙化软骨的虚拟岛是这些膨胀的横截面。计算机生成的3D重建清楚地表明，uncalcifiedepidencations通过钙化软骨接触骨和骨髓空间，并确认，软骨细胞的明显的埋葬是一个2D artefact.Conclusion：这项研究表明，软骨骨交界区比以前描述的更复杂。潮标是一个明确的边界，从钙化软骨中划分出未钙化软骨。它不是一条穿过关节的直线，而是一个复杂的三维结构，它沿着未钙化的软骨延伸，向下穿过钙化的软骨，邻接关节下的骨或骨髓空间。
Background: The chondro-osseous junctional region of diarthrodial joints is peculiarly complex and may be considered to consist of the deepest layer of non-calcified cartilage, the tidemark, the layer of calcified cartilage, a thin cement line ( between the calcified cartilage and the subchondral bone) and the subchondral bone. A detailed knowledge of the structure, function and pathophysiology of the normal chondroosseous junction is essential for an understanding of the pathogenesis of osteoarthrosis.Methods: Full thickness samples from human knee joints were processed and embedded in paraffin wax. One hundred serial sections ( 10 mu m thick) were taken from the chondro-osseous junctional region of a block from the medial tibial plateau of a normal joint. They were stained with haematoxylin and eosin and photographed. For a simple physical reconstruction images of each 10th sequential tissue section were printed and the areas of the photomicrographs containing the chondro-osseous junctional region were cut out and then overlaid so as to create a three-dimensional (3D) model of this region. A 3D reconstruction was also made using computer modelling.Results: Histochemical staining revealed some instances where prolongations of uncalcified cartilage, delineated by the tidemark, dipped into the calcified cartilage and, in places, abutted onto subchondral bone and marrow spaces. Small areas of uncalcified cartilage containing chondrocytes ( virtual islands) were seen, in two-dimensional (2D) sections, to be apparently entombed in calcified matrix. The simple physical 3D reconstruction confirmed that these prolongations of uncalcified cartilage were continuous with the cartilage of zone IV and demonstrated that the virtual islands of uncalcified cartilage were cross-sections of these prolongations. The computer-generated 3D reconstructions clearly demonstrated that the uncalcified prolongations ran through the calcified cartilage to touch bone and marrow spaces and confirmed that the apparent entombment of chondrocytes was a 2D artefact.Conclusion: This study demonstrates that the chondro-osseous junctional region is more complex than previously described. The tidemark is a clearly defined boundary delineating uncalcified from calcified cartilage. It is not a straight line across a joint, but a complex three-dimensional structure that follows uncalcified cartilage prolongations dipping down through the calcified cartilage to abut onto subjacent bone or marrow spaces.