Enhanced Detection of Single Viruses On-Chip via Hydrodynamic Focusing
Enhanced Detection of Single Viruses On-Chip via Hydrodynamic Focusing
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DOI：
10.1109/jstqe.2018.2854574
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发表时间：
2019-01-01
影响因子：
4.9
通讯作者：
Schmidt, Holger
中科院分区：
文献类型：
--
作者：
Black, Jennifer A.;Hamilton, Erik;Schmidt, Holger
平面光流控技术为促进芯片级光-物质相互作用提供了一个强有力的工具。硅基液芯波导已被证明可以提供单分子灵敏度，用于有效检测生物颗粒。最近，引入了基于PDMS的平面光流体平台，该平台利用基于二氧化硅的光流体和基于PDMS的微流体的积极属性，为独特结构的快速开发和原型制作开辟了道路。在这里，流体动力学聚焦集成到基于PDMS的光流体芯片中，以增强对芯片上单个H1N1病毒的检测。芯片平面聚焦由微流体通道系统提供，以迫使颗粒朝向高光学收集效率的区域。证明了聚焦，并使用荧光聚苯乙烯珠对增强的检测进行定量，其中发现随着流体动力学聚焦的加入，变异系数降低了4倍。发现荧光标记的单个H1N1病毒的平均信号幅度随着聚焦的增加而增加1.64倍。
Planar optofluidics provide a powerful tool for facilitating chip-scale light-matter interactions. Silicon-based liquid core waveguides have been shown to offer single molecule sensitivity for efficient detection of bioparticles. Recently, a PDMS based planar optofluidic platform was introduced that opens the way to rapid development and prototyping of unique structures, taking advantage of the positive attributes of silicon dioxide-based optofluidics and PDMS based microfluidics. Here, hydrodynamic focusing is integrated into a PDMS based optofluidic chip to enhance the detection of single H1N1 viruses on-chip. Chip-plane focusing is provided by a system of microfluidic channels to force the particles towards a region of high optical collection efficiency. Focusing is demonstrated and enhanced detection is quantified using fluorescent polystyrene beads where the coefficient of variation is found to decrease by a factor of 4 with the addition of hydrodynamic focusing. The mean signal amplitude of fluorescently tagged single H1N1 viruses is found to increase with the addition of focusing by a factor of 1.64.